Deconstruction of cellulosic fibers to fibrils based on enzymatic pretreatment
Deconstruction of cellulosic fibers to fibrils based on enzymatic pretreatment
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DOI：
10.1016/j.biortech.2018.07.067
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发表时间：
2018-11-01
影响因子：
11.4
通讯作者：
Xu, Jun
中科院分区：
文献类型：
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作者：
Wang, Shengdan;Gao, Wenhua;Xu, Jun
酶前处理在将纤维素纤维分解为原纤维方面显示出巨大的潜力，不仅经济有效，而且对环境友好。在这项研究中，一种广泛的商业内切葡聚糖酶被用来对纤维素纤维进行原纤化处理。用响应面方法优化了前处理时间和加酶量。当内切酶用量为9.0 mg/g(底物)，处理时间小于3.0h时，纤维回收率为100%，经0.5h处理后，纤维表面与天然纤维相比具有高度的原纤化和断裂性。同时，随着酶处理时间的延长，纤维素纤维逐渐解构。纤维的解构程度体现在纤维微观结构的变化上，如分子间/分子内氢键、β-1，4-糖苷键、结晶度和微晶尺寸等。这些发现为更有效、更经济地从纤维素纤维中分解原纤维的前处理方法提供了新的见解。
Enzymatic pretreatment has shown great potential in making the disintegration of cellulosic fibers to fibrils cost-effectively and environmental-friendly. In this study, an extensive commercial endoglucanase was used to pretreat cellulosic fibers for fibrillation. The pretreatment time and the enzyme dosage were optimized using response surface methodology. A 100% fiber recovery was obtained at endoglucanase usage of 9.0 mg/g (substrate) and pretreatment time of less than 3.0 h. A highly fibrillated and fractured surface of pretreated fibers was observed after 0.5 h of pretreatment compared to native fibers. Meanwhile, the progressive deconstruction of cellulosic fibers was occurred with the enzymatic pretreatment time increasing. The degree of deconstruction of fibers was evidenced by changes of the fiber microstructure, such as the inter-/intra-molecular H-bonds, the beta-1,4-glucosidic linkages, crystallinity and crystallite size. These discoveries provide new insights into a more efficient and economic pretreatment methods for the disintegration of fibrils from cellulosic fibers.