Purification of Egg White Lysozyme Determines the Downstream Fibrillation of Protein and Co-assembly with Phytochemicals to Form Edible Hydrogels Regulating the Lipid Metabolism.
Purification of Egg White Lysozyme Determines the Downstream Fibrillation of Protein and Co-assembly with Phytochemicals to Form Edible Hydrogels Regulating the Lipid Metabolism.
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DOI：
10.1021/acs.jafc.2c03363
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发表时间：
2022-07
影响因子：
6.1
通讯作者：
Xiaoqian He;Min Li;Yanhua Liu;Y. Nian;Bing Hu
中科院分区：
文献类型：
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作者：
Xiaoqian He;Min Li;Yanhua Liu;Y. Nian;Bing Hu
虽然合成化学或合成生物系统已经显示出生物材料工程的力量，但天然生物资源物质仍然是生产生物材料，特别是可食用生物材料的宝贵原料库。然而，上游的分离和纯化的原材料对它们的性能在下游加工过程中的影响仍然是未探索的，这是必不可少的生物材料的工程。在比较传统工艺的基础上，开发了一种简单、廉价的利用蛋清生产溶菌酶的方法-热诱导沉淀结合树脂共混离子交换法。即使在纯度相近的情况下，也只有该方法制备的溶菌酶才能形成有序的线状聚集原纤维。纤维化是近年来发展起来的一种利用生物资源生产高科技产品的新途径。植物化学物质完全取代盐，诱导溶菌酶原纤维自发形成水凝胶，这在体内研究中进一步证明，通过减少脂质吸收和脂肪生成，促进能量消耗和抑制炎症来预防由高脂饮食（HFD）诱导的肥胖。农业食品生物资源被成功地用作调节脂质代谢的可食用生物医学材料的概念验证。
Although the synthetic chemistry or synthetic biological systems have already shown the power of biomaterials engineering, natural bioresource matter is still a valuable library of raw ingredients for the production of biomaterials, in particular, the edible ones. However, the influence of upstream isolation and purification of the raw materials on their performance in the downstream processing procedures is still unexplored, which is essential for the engineering of biomaterials. Based on the comparison of conventional techniques, heating-induced precipitation combined with resin-blending ion exchange was developed as a simple and cheap method for the utilization of egg whites to produce the lysozyme that is found to be exclusively feasible for fibrillation. Even with similar purities, only the lysozyme prepared by this method could be utilized to form ordered linear aggregate fibrils. Fibrillation was recently pursued as a new approach to utilize bioresource mass for high-tech end-products. Phytochemicals, totally replacing salts, induced the lysozyme fibrils to form hydrogels spontaneously, which was further demonstrated in an in vivo study to prevent obesity induced by a high-fat diet (HFD) by reducing lipid absorption and lipogenesis, promoting energy expenditure, and inhibiting inflammation. The agri-food bioresource was successfully employed as a proof of concept in edible biomedical materials for the regulation of lipid metabolism.