The effect of helminth infection on the microbial composition and structure of the caprine abomasal microbiome.
The effect of helminth infection on the microbial composition and structure of the caprine abomasal microbiome.
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DOI：
10.1038/srep20606
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Urban JF
中科院分区：
文献类型：
--
作者：
Li RW;Li W;Sun J;Yu P;Baldwin RL;Urban JF
弯曲血蜱可以说是对小型反刍动物最有害的寄生虫。我们描述了扭曲螺旋杆菌感染对山羊皱胃微生物组的影响。用5000只弯纹夜蛾感染幼虫接种14只山羊，随访50 d。6只年龄匹配的naïve山羊作为未感染的对照。与未感染的对照组相比，感染山羊的体重增加减少，abosamal pH显著增加。感染也增加了细菌负荷，同时减少了皱胃中古生菌的丰度，但似乎没有影响微生物多样性。尽管如此，感染改变了每个样本检测到的432个物种级操作分类单位（OTU）中约19%的丰度。共有30个类群在对照山羊和感染山羊之间表现出显著的丰度差异。此外，感染导致微生物组结构的明显差异。多达8个KEGG通路被预测受到感染的显著影响。此外，H. contortus诱导的丁酸产生菌的改变可以调节粘膜炎症和组织修复。我们的研究结果为小反刍动物蠕虫感染的生理后果提供了见解，并有助于开发新的控制策略，以改善动物和人类的健康。
Haemonchus contortus is arguably the most injurious helminth parasite for small ruminants. We characterized the impact of H. contortus infection on the caprine abomasal microbiome. Fourteen parasite naive goats were inoculated with 5,000 H. contortus infective larvae and followed for 50 days. Six age-matched naïve goats served as uninfected controls. Reduced bodyweight gain and a significant increase in the abosamal pH was observed in infected goats compared to uninfected controls. Infection also increased the bacterial load while reducing the abundance of the Archaea in the abomasum but did not appear to affect microbial diversity. Nevertheless, the infection altered the abundance of approximately 19% of the 432 species-level operational taxonomic units (OTU) detected per sample. A total of 30 taxa displayed a significantly different abundance between control and infected goats. Furthermore, the infection resulted in a distinct difference in the microbiome structure. As many as 8 KEGG pathways were predicted to be significantly affected by infection. In addition, H. contortus-induced changes in butyrate producing bacteria could regulate mucosal inflammation and tissue repair. Our results provided insight into physiological consequences of helminth infection in small ruminants and could facilitate the development of novel control strategies to improve animal and human health.