EFFECT OF IMMUNIZATION ON THE EARLY INFLUX OF NEUTROPHILS DURING STAPHYLOCOCCAL MASTITIS IN EWES
EFFECT OF IMMUNIZATION ON THE EARLY INFLUX OF NEUTROPHILS DURING STAPHYLOCOCCAL MASTITIS IN EWES
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DOI：
10.1016/s0034-5288(18)32327-0
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发表时间：
1982-01-01
影响因子：
2.4
通讯作者：
WATSON, DL
中科院分区：
文献类型：
--
作者：
COLDITZ, IG;WATSON, DL
The neutrophil influx into mammary secretions was studied in unimmunized, and in systemically and locally immunized, lactating and non-lactating ewes experimentally infected with Staphylococcus aureus. Systemic immunization was effected by s.c. injection of live bacteria or by i.m. injection of killed bacteria in Freund''s incomplete adjuvant. The initial inflammatory response to infection of the mammary glands of systemically immunized lactating ewes was at first comparable with that for unimmunized, lactating ewes. By 8 h after challenge the leukocyte concentrations were lower in systemically immunized ewes. In non-lactating ewes immunized with live vaccine, higher leukocyte concentrations and higher proportions of neutrophils were recorded 4 h after infection (hpi) than for unimmunized ewes or ewes immunized with killed vaccine. Prior local immunization, by unilateral infusion of killed bacteria into mammary glands, enhanced the initial neutrophil influx in comparison to infection of unimmunized contralateral glands of non-lactating ewes by 4 hpi. For these animals the proportion of neutrophils in washings from immunized glands were significantly greater than for unimmunized glands 4 and 6 hpi. In 2 of 3 locally immunized lactating ewes there was a larger neutrophil influx into secretions of locally immunized glands than unimmunized glands 6 hpi. Neutrophils composed > 93% of leukocytes from all infected glands by 8 hpi. Evidently, differences in the rate of influx of neutrophils into infected mammary glands of immunized and unimmunized ewes could be attributed to immunological enhancement of neutrophil recruitment or to limitation of toxic damage to tissues with consequently diminished neutrophil invasion.
