Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3a
Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3a
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DOI：
10.1007/s007050050541
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发表时间：
1999-01-01
影响因子：
2.7
通讯作者：
Paton, DJ
中科院分区：
文献类型：
--
作者：
McGoldrick, A;Lowings, JP;Paton, DJ
对传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）分离株的分析表明，它们的嗜性和致病性受刺突蛋白和orf3的影响。一般来说，肠道病毒（TGEV）已被证明含有完整的spike和orf3基因，而呼吸道分离病毒（PRCV）在这两个区域都有重大缺失。毒性与orf3的功能有关。在这里，对最近分离的毒性TGEV的序列分析显示，一个突变具有完整的刺突基因，但ORF 3a有很大的缺失。这表明orf3a对肠道毒力并不是必需的。
Analyses of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) isolates have suggested that tropism and pathogenicity are influenced by the spike protein and ORF 3. In general, enteric viruses (TGEV) have been shown to contain intact spike and ORF 3 genes, whilst respiratory isolates (PRCV) have major deletions within both regions. Virulence has been correlated to a functional ORF 3. Here, sequence analysis of a recent isolate of virulent TGEV, revealed a variant with an intact spike gene, but a large deletion in ORF 3a. This suggests that ORF 3a is not essential for enteric virulence.