Localization of Sonic hedgehog secreting and receiving cells in the developing and adult rat adrenal cortex
Localization of Sonic hedgehog secreting and receiving cells in the developing and adult rat adrenal cortex
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DOI：
10.1016/j.mce.2010.11.010
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发表时间：
2011-04-10
影响因子：
4.1
通讯作者：
King, Peter
中科院分区：
文献类型：
--
作者：
Guasti, Leonardo;Paul, Alex;King, Peter
Sonic Hedgehog信号最近被证明在小鼠肾上腺皮质发育中发挥重要作用。大鼠肾上腺的组织结构与小鼠不同，大鼠的肾上腺球状带和束状带被未分化的区域隔开，而小鼠则不同。本研究旨在研究Sonic hedgehog及其信号通路组分Patch-1和Gill在发育中和成年大鼠肾上腺中的表达模式。在细胞色素P11B1和细胞色素P11B2表达缺失的细胞中，声波Hedgehog表达位于皮质周边，而信号接收细胞定位于被膜间充质。应用原位杂交和免疫组织化学相结合的方法，我们发现表达Sonic hedgehog的细胞位于细胞色素P11B2和细胞色素P11B1之间，因此Sonic Hedgehog的表达定义了未分化区的一个细胞群体。(C)2010爱思唯尔爱尔兰有限公司。保留所有权利。
Sonic hedgehog signaling was recently demonstrated to play an important role in murine adrenal cortex development. The organization of the rat adrenal differs from that of the mouse, with the zona glomerulosa and zona fasciculata separated by an undifferentiated zone in the rat, but not in the mouse. In the present study we aimed to determine the mRNA expression patterns of Sonic hedgehog and the hedgehog signaling pathway components Patched-1 and Gill in the developing and adult rat adrenal. Sonic hedgehog expression was detected at the periphery of the cortex in cells lacking CYP11B1 and CYP11B2 expression, while signal-receiving cells were localized in the overlying capsule mesenchyme. Using combined in situ hybridization and immunohistochemistry we found that the cells expressing Sonic hedgehog lie between the CYP11B2 and CYP11B1 layers, and thus Sonic hedgehog expression defines one cell population of the undifferentiated zone. (C) 2010 Elsevier Ireland Ltd. All rights reserved.