Visualizing the spatial relationships between defined DNA sequences and the axial region of extracted metaphase chromosomes
Visualizing the spatial relationships between defined DNA sequences and the axial region of extracted metaphase chromosomes
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DOI：
10.1016/s0092-8674(00)80996-4
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发表时间：
1996-01-12
期刊：
影响因子：
64.5
通讯作者：
Oghene, K
中科院分区：
文献类型：
--
作者：
Bickmore, WA;Oghene, K
使用荧光原位杂交提取的中期染色体，我们提出的视觉证据表明，特定的人类DNA序列占据独特的位置，相对于染色体的轴向区域和DNA组织成环从这个区域发出。在11号染色体上的一段独特的DNA中，可以追踪到DNA的大环，并且一个特定的区域与染色体的轴向区域相关。在rDNA中，非转录间隔区序列比rRNA基因更靠近染色体轴。异染色质和常染色质DNA似乎被组织成大小相似的环。我们不能检测到环在着丝粒;大多数alphoid DNA似乎仍然接近轴向区域。
Using fluorescence in situ hybridization to extracted metaphase chromosomes, we present visual evidence that specific human DNA sequences occupy distinctive positions with respect to the axial region of chromosomes and that the DNA is organized into loops emanating from this region. In a stretch of unique DNA on chromosome 11, large loops of DNA can be traced and one specific region associated with the axial region of the chromosome. Within rDNA, nontranscribed spacer sequences are more closely apposed to the chromosome axis than are rRNA genes. Heterochromatic and euchromatic DNAs appear to be organized into loops of similar size. We could not detect loops at centromeres; most alphoid DNA appears to remain close to the axial region.