Preparation of protein imprinted polymer beads by Pickering emulsion polymerization.
Preparation of protein imprinted polymer beads by Pickering emulsion polymerization.
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DOI：
10.1039/c4tb01605j
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
Tongchang Zhou;Ka Zhang;Tripta Kamra;L. Bülow;L. Ye
中科院分区：
文献类型：
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作者：
Tongchang Zhou;Ka Zhang;Tripta Kamra;L. Bülow;L. Ye
提出了一种基于Pickering乳液表面分子印迹的蛋白质特异性聚合物微球的制备方法。在第一步中，成人血红蛋白（Hb）吸附在二氧化硅纳米粒子。然后使用蛋白质涂覆的二氧化硅颗粒来稳定由油相中的交联单体组成的水包油乳液（皮克林乳液）。在油相的自由基聚合之后，去除蛋白质-二氧化硅颗粒以在聚合物表面上留下Hb印迹位点。用扫描电镜对印迹聚合物微球进行了表征，并通过蛋白结合分析考察了其选择性。基于Pickering乳液聚合的新合成方法在球形聚合物颗粒的表面产生了易于接近的Hb结合位点，这对于蛋白质的分离、纯化和分析是有用的。
We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.