Astrocyte mediated protection of fetal cerebral cortical neurons from rotenone and paraquat.
Astrocyte mediated protection of fetal cerebral cortical neurons from rotenone and paraquat.
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DOI：
10.1016/j.etap.2011.12.027
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发表时间：
2012-03
影响因子：
4.3
通讯作者：
Henderson GI
中科院分区：
文献类型：
--
作者：
Rathinam ML;Watts LT;Narasimhan M;Riar AK;Mahimainathan L;Henderson GI
原代培养的胎鼠皮层神经元和星形胶质细胞被用来测试的假设，星形胶质细胞介导的控制神经元谷胱甘肽（GSH）是一个有效的因素，对鱼藤酮和百草枯的神经保护。在神经元中，鱼藤酮（0.025至1μM）作用4小时和24小时会降低存活率，百草枯（2至100μM）也是如此。鱼藤酮（30 nM）使神经元活力和GSH降低24%和30%，而ROS增加56%。百草枯（30μM）使神经元活力和GSH分别降低36%和70%，而ROS则增加23%。当神经元与星形胶质细胞共培养时，其GSH在12和24 h分别增加1.5倍和5倍。与星形胶质细胞共培养可阻断鱼藤酮和百草枯引起的神经元死亡和损伤。星形胶质细胞介导的神经保护作用依赖于γ-谷氨酰循环组分的活性。这些研究阐明了星形胶质细胞介导的谷胱甘肽稳态对于保护神经元免受鱼藤酮和百草枯伤害的重要性，以及γ-谷氨酰循环在这种神经保护中的作用
Primary cultures of fetal rat cortical neurons and astrocytes were used to test the hypothesis that astrocyte-mediated control of neuronal glutathione (GSH) is a potent factor in neuroprotection against rotenone and paraquat. In neurons, rotenone (0.025 to 1μM) for 4 and 24 h decreased viability as did paraquat (2 to 100μM). Rotenone (30nM) decreased neuronal viability and GSH by 24% and 30%, while ROS were increased by 56%. Paraquat (30μM) decreased neuronal viability and GSH by 36% and 70%, while ROS were increased by 23%. When neurons were co-cultured with astrocytes, their GSH increased 1.5 fold and 5 fold at 12 and 24 h. Co-culturing with astrocytes blocked neuronal death and damage by rotenone and paraquat. Astrocyte-mediated neuroprotection was dependent on the activity of components of the γ-glutamyl cycle. These studies illustrate the importance of astrocyte-mediated glutathione homeostasis for protection of neurons from rotenone and paraquat and the role of the γ-glutamyl cycle in this neuroprotection
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DOI：
10.1097/nen.0b013e3181db8100
发表时间：
2010-05
影响因子：
3.2
作者：
Choi WS;Abel G;Klintworth H;Flavell RA;Xia Z
通讯作者：
Xia Z
DOI：
10.1083/jcb.85.3.890
发表时间：
1980-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
McCarthy KD;de Vellis J
通讯作者：
de Vellis J
影响因子：
4.7
作者：
Ju, C;Yoon, KN;Kim, WK
通讯作者：
Kim, WK
影响因子：
2.5
作者：
Kranich, O;Hamprecht, B;Dringen, R
通讯作者：
Dringen, R
影响因子：
4.7
作者：
McCormack, AL;Di Monte, DA
通讯作者：
Di Monte, DA