Deep Cap Analysis of Gene Expression
Deep Cap Analysis of Gene Expression
复制标题
DOI：
10.1007/978-1-60761-944-4_10
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Hayashizaki, Yoshihide
中科院分区：
文献类型：
--
作者：
Kurosawa, Junpei;Nishiyori, Hiromi;Hayashizaki, Yoshihide
建立了基因表达的cap分析（CAGE）技术，利用5' cDNA标记和PCR检测转录起始位点（tss）和表达水平。据报道，模板的数量与PCR的扩增效率成正比，CAGE已被用作分析启动子活性和发现新转录物（包括替代剪接产物和非编码转录物）的关键技术。在这里，我们介绍了更强大的工具，如deepCAGE，可用于高通量下一代测序技术。DeepCAGE可以产生更深入的转录组数据集，并可以揭示调控网络的更多细节。
The cap analysis of gene expression (CAGE) technology has been established to detect transcriptional starting sites (TSSs) and expression levels by utilizing 5' cDNA tags and PCR. It has been reported that the amount of templates is proportional to the amplification efficiency of PCR CAGE has been used as a key technique for analyzing promoter activity and finding new transcripts including alternative spliced products and noncoding transcripts. Here, we introduce more powerful tools such as deepCAGE, which can be utilized for high-throughput next-generation sequencing technology. DeepCAGE can produce much deeper transcriptome datasets and can reveal more details of the regulatory network.