Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets
Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets
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DOI：
10.2334/josnusd.18-0143
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发表时间：
2019-06-01
影响因子：
1.9
通讯作者：
Honda, Masaki
中科院分区：
文献类型：
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作者：
Mashimo, Takayuki;Sato, Yukio;Honda, Masaki
骨髓间充质干细胞是骨组织工程研究中应用最广泛的成骨细胞来源。作为骨移植的替代方法，基于细胞的牙槽嵴增高治疗已受到关注。在本研究中，BMMSC移植到C57 BL/6 J小鼠拔牙窝中的牙槽嵴再生进行了评价。拔除右侧上颌第一磨牙，然后立即将从股骨和胫骨骨髓中分离的BMMSCs（PDGFR alpha（+）Sca-1（+）CD 45（-）TER 119（-）细胞）移植到拔牙窝中。对照组除行骨髓间充质干细胞移植外，其余均行相同的手术。使用显微计算机断层扫描、组织学和免疫组织化学分析评价牙槽骨形成。在3周时，实验组的窝中骨形成比对照组更先进。移植后6周的组织学分析表明，实验组的窝也含有更多的骨髓。有趣的是，在6周时，实验组的牙槽骨矿物质密度低于对照组。这些结果表明，骨髓间充质干细胞移植加速骨愈合和增加骨髓形成拔牙窝。
Bone marrow-derived mesenchymal stem cells (BMMSCs) remain the most widely used source of osteogenic cells in bone tissue engineering research. A cell-based treatment for alveolar ridge augmentation has received attention as an alternative to bone grafting. In the present study, BMMSC transplantation into tooth extraction sockets of C57BL/6J mice was evaluated for alveolar ridge regeneration. The first right maxillary molars were extracted, and then BMMSCs (PDGFR alpha(+) Sca-1(+) CD45(-) TER119(-) cells) isolated from femoral and tibial bone marrow were immediately transplanted into the extraction sockets. A control group underwent the same procedure except for BMMSC transplantation. Bone formation in the sockets was evaluated using micro-computed tomography and histological and immunohistochemical analyses. At 3 weeks, bone formation in the sockets was more advanced in the experimental group than in the control group. Histological analysis at 6 weeks after transplantation showed that the sockets in the experimental group also contained a greater quantity of bone marrow. Interestingly, socket bone mineral density was lower in the experimental group than in the control group at 6 weeks. These findings suggest that BMMSC transplantation accelerates bone healing and augments bone marrow formation in tooth extraction sockets.