Light-Mediated Spatial Control via Photolabile Fluorescently Quenched Peptide Cassettes
Light-Mediated Spatial Control via Photolabile Fluorescently Quenched Peptide Cassettes
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DOI：
10.1021/ja907427p
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发表时间：
2010-02-10
影响因子：
15
通讯作者：
Lawrence, David S.
中科院分区：
文献类型：
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作者：
Lee, Hsien-Ming;Priestman, Melanie A.;Lawrence, David S.
作为生物行为的空间和时间探针，光调节化合物的效用越来越大。当生物探针的改变（例如，激活，失活等）可以直接与荧光读数联系起来时，成功的光诱导结构变化的独立测量是可能的。我们已经确定了一系列的光不稳定性荧光猝灭磁带，显示非常大的荧光增强在光解。一对磁带被插入到线粒体定位序列中，以评估控制生物活性的器官I I I e靶向光介导释放策略。肽结构很容易被线粒体吸收，随后可以通过预测的吸光度和荧光变化来评估以光依赖的方式切割。
Light-regulatable compounds are finding increasing utility as spatial and temporal probes of biological behavior. An independent measure Of Successful light-induced structural change is possible when alteration (e.g., activation, deactivation, etc.) of the bioprobe can be directly linked to a fluorescent readout. We have identified a series of photolabile fluorescently quenched cassettes that display extraordinarily large fluorescence enhancements upon photolysis. A pair of cassettes has been inserted into mitochondrial localization sequences to assess an organ e I I e-targeted light-mediated release strategy for controlling biological activity. The peptide constructs are readily absorbed by mitochondria and subsequently can be cleaved in a light-dependent fashion as assessed by the predicted changes in absorbance and fluorescence.