Wolbachia affects reproduction in the spider mite Tetranychus truncatus (Acari: Tetranychidae) by regulating chorion protein S38 ‐like and Rop
Wolbachia affects reproduction in the spider mite Tetranychus truncatus (Acari: Tetranychidae) by regulating chorion protein S38 ‐like and Rop
复制标题
沃尔巴克氏体通过调节绒毛膜蛋白 S38 —like 和 Rop 影响叶螨 Tetranychus truncatus（螨虫：Tetranychidae）的繁殖
DOI：
10.1111/imb.12669
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发表时间：
2020
影响因子：
2.6
通讯作者：
X.‐Y. Hong
中科院分区：
文献类型：
--
作者：
X. Xia;C.‐W. Peng;J.‐R. Cui;P.‐Y. Jin;K. Yang;X.‐Y. Hong
Wolbachia诱导的宿主生殖调控已被用于控制害虫种群，但对宿主基因Wolbachia调控的分子机制知之甚少。本文从分子水平上研究了Wolbachia对截形叶螨（Tetranychus truncatus）的生殖调控作用。进一步的RNA序列分析显示了受WolbachiainT调控的基因。 truncatus)(采自菜豆)。真实的定量聚合酶链反应（qPCR）显示，包括绒毛膜蛋白S38‐ like和Rop在内的基因在Wolbachia的作用下下调。利用RNA干扰技术（RNAi）对未感染沃尔巴克氏菌的T.截尾虫减少产卵，这与沃尔巴克氏菌诱导的产卵减少一致。有趣的是，抑制罗平氏菌感染的T.截短导致卵中Wolbachiattres增加;然而，在RNAi绒毛膜蛋白S38‐like后，这种情况没有发生。这是第一个研究表明绒毛膜蛋白S38样蛋白和Rop促进了沃尔巴克氏菌介导的截形毛滴虫生育力的变化。此外，Rop基因的RNAi可以改变未感染Wolbachia的T. Truncatusblack，表明Rop的作用不仅限于对T. truncatus)(采自菜豆)。
Wolbachia‐induced reproductive regulation in hosts has been used to control pest populations, but little is known about the molecular mechanism underlyingWolbachiaregulation of host genes. Here, reproductive regulation byWolbachiain the spider miteTetranychus truncatuswas studied at the molecular level. Infection withWolbachiaresulted in decreasing oviposition and cytoplasmic incompatibility inT. truncatus.Further RNA‐seq revealed genes regulated byWolbachiainT. truncatus. Real‐time quantitative polymerase chain reaction (qPCR) showed that genes, includingchorion protein S38‐likeandRopwere down‐regulated byWolbachia. RNA interference (RNAi) ofchorion protein S38‐likeandRopinWolbachia‐uninfectedT. truncatusdecreased oviposition, which was consistent withWolbachia‐induced oviposition decrease. Interestingly, suppressingRopinWolbachia‐infectedT. truncatusled to increasedWolbachiatitres in eggs; however, this did not occur after RNAi ofchorion protein S38‐like. This is the first study to show thatchorion protein S38‐likeandRopfacilitateWolbachia‐mediated changes inT. truncatusfertility. In addition, RNAi ofRopturned the body colour ofWolbachia‐uninfectedT. truncatusblack, which indicates that the role ofRopis not limited to the reproductive regulation ofT. truncatus.