Dominance of two genotypes of Bordetella pertussis during a period of increased pertussis activity in Alberta, Canada: January to August 2012
Dominance of two genotypes of Bordetella pertussis during a period of increased pertussis activity in Alberta, Canada: January to August 2012
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DOI：
10.1016/j.ijid.2014.10.028
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发表时间：
2014-12-01
影响因子：
8.4
通讯作者：
Drews, Steven J.
中科院分区：
文献类型：
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作者：
Simmonds, Kimberley;Fathima, Sumana;Drews, Steven J.
目的：本研究的目的是对加拿大阿尔伯塔2012年1月1日至8月31日期间百日咳杆菌活动增加进行流行病学分析。B。百日咳检测使用IS 481实时PCR检测试剂盒进行，对PCR阳性和不确定标本进行培养和储存以供进一步分析。方法：实验室数据与传染病报告系统（CDRS）中报告的阿尔伯塔卫生部（AH）病例相关联，以识别病例分离株;使用排除标准来避免偏见。在国家微生物实验室（NML）通过脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）分析病例分离株。百日咳免疫数据提取自阿尔伯塔省免疫库（Imm/ARI）并与百日咳病例相关联。结果：使用PFGE和MLST，52例病例分离株可分为两个主要序列类型组：41例属于ST-1组（ST-1和新型ST-19），11例属于ST-2组（ST-2和新型ST-20）。在基因分型的总病例（N = 52）中，18例（34.6%）有免疫史，28例（53.8%）未免疫，6例（11.6%）免疫史不详。在所有未免疫的病例中，25/28例（89.2%）属于ST-1组。此外，41 ST-1组的情况下，25个没有免疫相比，只有三个ST-2组的情况下（p = 0.0004，Fisher精确检验）。结论：这项研究显示了两种基因型的B的优势。在我们的管辖范围内的百日咳，并表明与ST-1组感染的个人较少百日咳免疫。(C)2014作者由爱思唯尔有限公司代表国际传染病学会出版。
Objectives: The purpose of this study was to undertake an epidemiological analysis of an increase in Bordetella pertussis activity during the period January 1 to August 31, 2012 in Alberta, Canada. B. pertussis testing was done using an IS481 real-time PCR assay with PCR-positive and indeterminate specimens cultured and stored for further analysis.Methods: Laboratory data were linked to Alberta Health (AH) cases that were reported in the Communicable Disease Reporting System (CDRS) to identify case isolates; exclusion criteria were used to avoid biases. Case isolates were analyzed at the National Microbiology Laboratory (NML) by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pertussis immunization data were extracted from the Alberta Provincial Immunization Repository (Imm/ARI) and linked to the pertussis cases.Results: Using PFGE and MLST, 52 case isolates could be divided into two main sequence type groups: 41 cases belonged to the ST-1 group (ST-1 and the novel ST-19) and 11 cases belonged to the ST-2 group (ST-2 and the novel ST-20). Of the total cases genotyped (N = 52), 18 (34.6%) had a history of immunization, 28 (53.8%) were not immunized, and six (11.6%) had an unknown immunization history. Of the total non-immunized cases, 25/28 (89.2%) belonged to the ST-1 group. Furthermore, of the 41 ST-1 group cases, 25 were not immunized compared to only three of the ST-2 group cases (p = 0.0004, Fisher's exact test).Conclusions: This study shows the dominance of two genotypes of B. pertussis in our jurisdiction and indicates less pertussis immunization in individuals infected with the ST-1 group. (C) 2014 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.