High serum levels of proinflammatory markers during epileptogenesis. Can omega-3 fatty acid administration reduce this process?
High serum levels of proinflammatory markers during epileptogenesis. Can omega-3 fatty acid administration reduce this process?
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DOI：
10.1016/j.yebeh.2015.07.021
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发表时间：
2015-10-01
影响因子：
2.6
通讯作者：
Naffah-Mazzacoratti, Maria da Graca
中科院分区：
文献类型：
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作者：
Furtado Gouveia, Telma Luciana;Vieira de Sousa, Paula Viviane;Naffah-Mazzacoratti, Maria da Graca
在癫痫过程中，可能会发生几种事件，如中枢神经系统中免疫系统的重要激活。对癫痫发作活动的反应导致大脑和外周的炎症。此外，CRP和细胞因子可能能够与许多配体相互作用，以响应由发作和发作后状态中的交感神经刺激引起的心脏损伤。在此基础上，我们测量了血清中的C-反应蛋白（CRP）和细胞因子在急性，沉默，和慢性阶段提交给匹鲁卡品癫痫模型的大鼠。我们还分析了在癫痫灶生成期间，用ω-3脂肪酸对这些大鼠进行慢性治疗对CRP和细胞因子水平的影响。C-反应蛋白和细胞因子如IL-1 β、IL-6和TNF-α在大鼠血液中呈现高浓度，即使在SE发生后也是如此。我们发现，与用溶剂溶液治疗的动物相比，用omega-3治疗的慢性癫痫动物血液中CRP和所有促炎细胞因子水平降低。综上所述，我们的结果强烈表明，ω-3是一种有效的治疗，以防止SUDEP的发生，因为它的能力，作为一种抗炎化合物，减少癫痫发作改变全身炎症参数。(c)2015 Elsevier Inc. All rights reserved.
During the epileptogenic process, several events may occur, such as an important activation of the immune system in the central nervous system. The response to seizure activity results in an inflammation in the brain as well as in the periphery. Moreover, CRP and cytokines may be able to interact with numerous ligands in response to cardiac injury caused by sympathetic stimulation in ictal and postictal states. Based on this, we measured the serum levels of C-reactive protein (CRP) and cytokines during acute, silent, and chronic phases of rats submitted to the pilocarpine model of epilepsy. We have also analyzed the effect of a chronic treatment of these rats with omega-3 fatty acid in CRP and cytokine levels, during an epileptic focus generation. C-reactive protein and cytokines such as IL-1 beta, IL-6, and TNF-alpha presented high concentration in the blood of rats, even well after the occurrence of SE. We found reduced levels of CRP and all proinflammatory cytokines in the blood of animals with chronic seizures, treated with omega-3, when compared with those treated with vehicle solution. Taken together, our results strongly suggest that the omega-3 is an effective treatment to prevent SUDEP occurrence due to its capability to act as an anti-inflammatory compound, reducing the systemic inflammatory parameters altered by seizures. (c) 2015 Elsevier Inc. All rights reserved.