The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa.
The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa.
复制标题
从酿酒酵母和粗糙脉孢菌中提取含肌醇的磷脂和磷脂酰胆碱。
作者：
B. Hanson;R. Lester
通过使用在[3 H]-肌醇和[14 C]胆碱存在下生长的真菌，我们探索了定量提取含肌醇的磷脂和磷脂酰胆碱的方法。乙醇-水和乙醇-二乙醚-水的微碱性混合物在升高的温度下显示出有效地从完整的酿酒酵母和粗糙脉孢菌中提取这些脂质。一些先前公布的程序未能完全提取这些生物体的极极性含磷酸肌醇的鞘脂。在提取前，可谨慎使用三氯乙酸杀死细胞;在升高的浓度和温度下可发生脂质破坏。用极性溶剂完全提取这些极性很强的脂质也会导致提取物含有大量的非脂质。
By use of fungi grown in the presence of [3H]-inositol and [14C]choline, we have explored methods for the quantitative extraction of inositol-containing phospholipids and phosphatidylcholine. Slightly alkaline mixtures of both ethanol-water and ethanol-diethylether-water at elevated temperatures were shown to effectively extract these lipids from intact Saccharomyces cerevisiae and Neurospora crassa. Some previously published procedures fail to completely extract to very polar phosphoinositol-containing sphingolipids of these organisms. Trichloroacetic acid can be used with caution in killing cells prior to extraction; lipid destruction can occur at elevated concentrations and temperatures. Complete extraction of these very polar lipids with polar solvents also results in an extract containing significant amounts of non-lipids.