Opioidergic Interactions between Striatal Projection Neurons
Opioidergic Interactions between Striatal Projection Neurons
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DOI：
10.1523/jneurosci.1775-11.2011
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发表时间：
2011-09-21
影响因子：
5.3
通讯作者：
Bracci, Enrico
中科院分区：
文献类型：
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作者：
Blomeley, Craig P.;Bracci, Enrico
中型棘状纹状体投射神经元（MSN）释放阿片类神经肽，但这些神经递质的作用仍然知之甚少。虽然已经使用外源性配体证明了阿片受体对皮质纹状体轴突的突触前抑制，但尚未研究纹状体中突触释放的阿片样物质的作用。我们进行了单和成对的全细胞记录大鼠MSN，而皮质纹状体纤维被电激活。在单记录实验中，我们还逆向激活了MSN群体的轴突。皮质纹状体纤维每10秒刺激一次，每隔一次刺激之前有5个逆向尖峰（100 Hz）。这种逆向尖峰的爆发产生了强烈的抑制诱发皮质纹状体反应。这种抑制作用不受δ-阿片受体拮抗剂SDM 25 N的影响，但被μ-阿片受体拮抗剂CTOP完全消除。当爆发先于皮质纹状体刺激500 ms时，抑制作用最大（平均29.6 +/- 11.4%），并且在间隔>2 s时变得不可检测。来自MSN的配对录音位于
Medium spiny striatal projection neurons (MSNs) release opioid neuropeptides, but the role of these neurotransmitters is still poorly understood. While presynaptic inhibition of corticostriatal axons by opioid receptors has been demonstrated using exogenous ligands, the action of synaptically released opioids in the striatum has not been investigated. We performed single and paired whole-cell recordings from rat MSNs while corticostriatal fibers were electrically activated. In single recording experiments, we also activated antidromically the axons of a population of MSNs. Corticostriatal fibers were stimulated once every 10 s and every other stimulation was preceded by 5 antidromic spikes (at 100 Hz). This burst of antidromic spikes produced robust inhibition of evoked corticostriatal responses. This inhibition was not affected by the delta-opioid receptor antagonist SDM25N, but was completely abolished by the mu-opioid receptor antagonist CTOP. Inhibitory effects were maximal (on average 29.6 +/- 11.4%) when the burst preceded the corticostriatal stimulation by 500 ms and became undetectable for intervals >2 s. Paired recordings from MSNs located