Molecular investigation of hard ticks (Acari: Ixodidae) and fleas (Siphonaptera: Pulicidae) as potential vectors of rickettsial and mycoplasmal agents
Molecular investigation of hard ticks (Acari: Ixodidae) and fleas (Siphonaptera: Pulicidae) as potential vectors of rickettsial and mycoplasmal agents
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DOI：
10.1016/j.vetmic.2009.07.013
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发表时间：
2010-01-06
影响因子：
3.3
通讯作者：
Hofmann-Lehmann, Regina
中科院分区：
文献类型：
--
作者：
Hornok, Sandor;Meli, Marina L.;Hofmann-Lehmann, Regina
本研究报告有两个目的。首先，在一般情况下，揭示新的方面的潜在的媒介作用的硬蜱和跳蚤，通过筛选大量的标本与最近开发的分子生物学方法。第二，评估地理环境中病媒传播的传染性病原体的发生情况。共收集未取食硬蜱3442只（篦齿硬蜱、缘革蜱、革蜱）。reticulatus、Haemaphysalis inermis、H. concinna，H. punctata）和猫、狗蚤939只（Ctenocephalides felis，C.犬蚤（Pulex irritans）。提取DNA并在池中分析立克次体目和支原体目的代表。inermis是新确定的最重要的潜在载体的研究区域的瑞士立克次体。一种新的立克次体基因型（命名为“Rickettus R.在相同的蜱种中也检测到了gltA基因，与已知的立克次体的gltA基因序列最高有95.8%的同一性。此外，刺激巴斯德氏菌对立克次氏体RF2125检测呈阳性，这在欧洲以前没有描述过。人类病原体R.猫和猫的病原体'Mycoplasma turicensis'首次出现在中欧和东欧。进一步的新发现包括螺原体属的存在。in D.边缘虫和跳蚤总之，这项分子研究扩展了几种节肢动物传播的媒介的地理范围和矢量谱，其中一些具有人畜共患病的潜力。(C)2009爱思唯尔有限公司版权所有。
The aim of the present study was twofold. First, in general, to reveal new aspects of the potential vector role of ixodid ticks and fleas by screening large numbers of specimens with recently developed molecular biological methods. Second, to evaluate the occurrence of vector-borne infectious agents in a geographical context. Altogether 3442 unfed hard ticks (Ixodes ricinus, Dermacentor marginatus, D. reticulatus, Haemaphysalis inermis, H. concinna, H. punctata) and 939 fleas of cats and dogs (Ctenocephalides felis, C. canis, Pulex irritans) were collected in Hungary. DNA was extracted and analyzed in pools for representatives of the orders Rickettsiales and Mycoplasmatales.H. inermis was newly identified as the most important potential vector for Rickettsia helvetica in the study region. A novel Rickettsia genotype (designated 'Candidatus R. hungarica') was also detected in the same tick species, with a maximum of 95.8% gltA gene sequence identity to known rickettsiae. In addition, P. irritans tested positive for Rickettsia sp. RF2125, which has not been previously described in Europe. The human pathogen R. felis and the feline pathogen 'Candidatus Mycoplasma turicensis' were shown for the first time to occur in Central-Eastern Europe. Further novel findings include the presence of Spiroplasma spp. in D. marginatus and fleas. In conclusion, this molecular study extends the geographic range and vector spectrum of several arthropod-borne agents, some of which have zoonotic potential. (C) 2009 Elsevier B.V. All rights reserved.