Immunolocalisation of thrombospondin 1 in human, bovine and rabbit cornea
Immunolocalisation of thrombospondin 1 in human, bovine and rabbit cornea
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DOI：
10.1007/s004410050877
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发表时间：
1997-08-01
影响因子：
3.6
通讯作者：
Naumann, GOH
中科院分区：
文献类型：
--
作者：
Hiscott, P;Seitz, B;Naumann, GOH
用光镜和电子显微镜免疫组织化学技术研究了基质细胞蛋白凝血酶原蛋白1在正常人、牛和兔角膜中的分布。光镜下观察了人和牛角膜上皮基底膜、后后弹力膜和内皮细胞中凝血酶反应蛋白1的免疫反应。人和牛角膜的基质、基质细胞(角质细胞)和Descemet膜的前部均未检测到凝血酶反应蛋白1，兔角膜各层均未检测到该蛋白。人和牛角膜的电子显微镜免疫金研究表明，角膜内皮(和基底上皮)细胞的凝血酶反应蛋白1标记包括细胞膜上的灶性沉积。据推测，在正常人和牛的角膜内皮和基底上皮细胞中，凝血酶反应蛋白1调节细胞与基底膜之间的相互作用，也可能调节细胞与细胞之间的相互作用。
Light- and electron-microscopic immunohistochemical techniques were used to investigate the distribution of the matricellular protein thrombospondin 1 in normal human, bovine and rabbit cornea. Light-microscopic immunoreactivity for thrombospondin 1 was observed in the epithelial basement membrane, posterior Descemet's membrane and endothelium of human and bovine cornea. The bulk of the stroma, the stromal cells (keratocytes) and the anterior part of Descemet's membrane in human and bovine cornea were devoid of detectable thrombospondin 1 and the protein could not be demonstrated in any of the layers of the rabbit cornea. Electron-microscopic immunogold studies of human and bovine cornea revealed that thrombospondin 1 labelling of corneal endothelial (and basal epithelial) cells included focal deposits at cell membranes. It is postulated that thrombospondin 1 regulates interactions between cells and their basement membrane, and perhaps cell-to-cell interactions, in the normal human and bovine corneal endothelium and basal epithelium.