Preparation of photoreactive derivatives of alpha-melanotropin by selective modification of the lysine or tryptophan residue.
Preparation of photoreactive derivatives of alpha-melanotropin by selective modification of the lysine or tryptophan residue.
复制标题
通过选择性修饰赖氨酸或色氨酸残基制备α-促黑素激素的光反应衍生物。
DOI：
10.1111/j.1399-3011.1982.tb00901.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Ramachandran,J
中科院分区：
文献类型：
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作者：
Muramoto,K;Buckley,DI;Ramachandran,J
通过与2-硝基-4-叠氮基苯磺酰氯反应，选择性修饰9位的单个色氨酸残基或11位赖氨酸残基的氨基，制备α-促黑素素的光反应性衍生物。发现[2-硝基-4-叠氮苯硫基-Trp 9]-α-促黑素在刺激分离的兔脂肪细胞中的脂解方面的效力是α-促黑素的5倍。[Nε-2-硝基-4-叠氮基苯硫基-Lys 11]-α-促黑激素的脂解效力仅为α-促黑激素的11%。
Photoreactive derivatives of α‐melanotropin were prepared by selective modification of the single tryptophan residue in position 9 or the amino group of the lysine residue in position 11 by reaction with 2‐nitro‐4‐azidophenylsulfenyl chloride. [2‐Nitro‐4‐azidophenylsulfenyl‐Trp9]‐α‐melanotropin was found to be five times as potent as α‐melanotropin in stimulating lipolysis in isolated rabbit adipocytes. [Nε‐2‐nitro‐4‐azidophenylsulfenyl‐Lys11]‐α‐melanotropin had only 11% of the lipolytic potency of α‐melanotropin.