An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis
An investigation of hormesis of trichloroethylene in L-02 liver cells by differential proteomic analysis
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差异蛋白质组分析研究L-02肝细胞中三氯乙烯的兴奋作用
DOI：
10.1007/s11033-008-9424-z
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Zhuang, Zhi-Xiong
中科院分区：
文献类型：
--
作者：
Huang, Hai-Yan;Liu, Jian-Jun;Zhuang, Zhi-Xiong
毒物兴奋效应是生物对有毒化学物质反应的剂量-反应模式，以低剂量刺激和高剂量抑制为特征。尽管已知某些细胞类型对低水平的细胞毒性剂表现出适应性反应，但其分子机制仍不清楚，并且尚未确定这是否是所有细胞类型对暴露于每种化学品的反应中发生的普遍现象。三氯乙烯（TCE）是一种广泛使用的有机溶剂，在工业生产过程中会释放到大气中。三氯乙烯急性（短期）和慢性（长期）吸入暴露可影响人体健康。为了阐明L-02肝细胞暴露于低剂量TCE后的细胞生存适应性反应，采用CCK-8法评估细胞毒性，并通过蛋白质组学技术研究毒物兴奋效应的可能机制。结果发现，L-02肝细胞在低浓度TCE暴露后，对高浓度TCE暴露产生了适应性变化，双向电泳得到约1,000个蛋白质点，基质辅助激光解吸电离质谱和胰蛋白酶肽段串联质谱测序鉴定出5个蛋白质点。本研究结果提示，TCE诱导的兴奋效应可能与所鉴定的蛋白质有关，这对进一步研究TCE的作用机制和风险评估具有重要意义。
Hormesis is the dose-response pattern of the biological responses to toxic chemicals, characterized by low-dose stimulation and high-dose inhibition. Although it is known that some cell types exhibit an adaptive response to low levels of cytotoxic agents, its molecular mechanism is still unclear and it has yet to be established whether this is a universal phenomenon that occurs in all cell types in response to exposure to every chemical. Trichloroethylene (TCE) is an organic solvent widely used and is released into the atmosphere from industrial degreasing operations. Acute (short-term) and chronic (long-term) inhalation exposure to trichloroethylene can affect the human health. In order to elucidate a cell-survival adaptive response of L-02 liver cells exposed to low dose of TCE, CCK-8 assay was used to assess cytotoxicity, and examined the possible mechanisms of hormesis by proteomics technology. We found that exposure of L-02 liver cells to low level of TCE resulted in adaptation to further exposure to higher level, about 1,000 protein-spots were obtained by two-dimensional electrophoresis (2-DE) and five protein spots were identified by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry sequencing of tryptic peptides. Our results suggest that a relationship may exist between identified proteins and TCE-induced hormesis, which are very useful for further study of the mechanism and risk assessment of TCE.