Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment
Generation and application of a novel transgenic zebrafish line Tg(cyp1a:mCherry) as an in vivo assay to sensitively monitor PAHs and TCDD in the environment
复制标题
新型转基因斑马鱼系 Tg(cyp1a:mCherry) 的生成和应用作为灵敏监测环境中 PAH 和 TCDD 的体内检测
DOI：
10.1016/j.jhazmat.2017.11.021
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Ma yanbo
中科院分区：
文献类型：
--
作者：
Xie shaolin;Junaid Muhammad;Bian wanping;Luo juanjuan;Syed Jabir Hussain;Wang chao;Xiong wenxu;Ma yanbo
多环芳烃(PAHs)和2，3，7，8-四氯二苯并对二恶英(TCDD)被列为人类致癌物质，并可造成严重的健康问题。为了开发一种方便的生物监测工具来检测环境中的PAHs和TCDD，我们构建了一个含有cyp1a启动子驱动mCherry表达的转基因斑马鱼品系TG(cyp1a：mCherry)。在这里，TG(cyp1a：mCherry)胚胎被不同浓度的TCDD和5个美国环保局优先考虑的多环芳烃同系物处理。结果表明，mCherry和内源cyp1a的表达与多环芳烃的暴露浓度一致，主要受TCDD和≥4环多环芳烃的诱导。此外，还通过监测水和土壤样品中的多环芳烃污染来评估Tg(cyp1a：mCherry)胚胎的敏感性。Tg(Cyp1a：mCherry)斑马鱼暴露于高浓度≥四环多环芳烃的水样和土壤有机提取物后，红色荧光信号和cyp1a表达水平均升高。这些结果进一步加强了我们对新建立的斑马鱼的浓度和同系物依赖反应的发现。综上所述，新建立的斑马鱼系列将被证明是监测环境中多环芳烃和TCDD污染的敏感、高效和方便的工具。
The polycyclic aromatic hydrocarbons (PAHs) and 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) are classified as human carcinogens, and can also cause serious health problems. To develop a convenient bio-monitoring tool for the detection of PAHs and TCDD in the environment, we generated a transgenic zebrafish line Tg(cyp1a:mCherry) with cyp1a promoter driving mCherry expression. Here, Tg(cyp1a:mCherry) embryos were treated with different concentrations of TCDD and five US EPA priority PAHs congeners. The results showed that the expressions of mCherry and endogenous cyp1a were consistent with the PAHs exposure concentrations and were largely induced by TCDD and ≥4-ring PAHs. Moreover, the sensitivity of Tg(cyp1a:mCherry) embryos was also evaluated through monitoring of the PAHs contamination in the water and soil samples. The elevated red fluorescent signals and cyp1a expression levels were observed in Tg(cyp1a:mCherry) zebrafish after exposure to water samples and soil organic extracts with higher concentrations of ≥4-ring PAHs. These results further strengthen our findings of concentration- and congener-dependent response of the newly established zebrafish. Taken together, the newly established zebrafish line will prove as a sensitive, efficient and convenient tool for monitoring PAHs and TCDD contamination in the environment