Brain atlas of the Mongolian gerbil (Meriones unguiculatus) in CT/MRI-aided stereotaxic coordinates.
Brain atlas of the Mongolian gerbil (Meriones unguiculatus) in CT/MRI-aided stereotaxic coordinates.
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DOI：
10.1007/s00429-016-1259-0
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Budinger E
中科院分区：
文献类型：
--
作者：
Radtke-Schuller S;Schuller G;Angenstein F;Grosser OS;Goldschmidt J;Budinger E
长爪沙鼠是神经科学中的重要动物模型，本文报道了一种新的长爪沙鼠脑立体定位图谱。它将高质量的组织学材料与可靠的立体定位坐标相结合，用于识别大脑结构。该图谱由62个吻尾侧水平的额叶切片的高分辨率图像组成，细胞体（Nissl）和有髓纤维（Gallyas）以350 μm的间隔交替染色。大脑结构根据Paxinos啮齿动物命名法命名。通过将组织切片系列与通过磁共振成像（MRI）获得的沙鼠体内脑图像进行比较和匹配，大大提高了立体定位坐标系的准确性。使用X射线计算机断层扫描（CT）获得与MR图像相对应的颅骨轮廓，并用于建立脑结构和颅骨坐标之间的关系。诸如lambda、前囟、耳道和枕骨嵴的标志可以用于在标准图谱坐标中排列头骨和大脑。一个容易重现的协议允许切片的实验大脑在标准的额状面的图集。
A new stereotaxic brain atlas of the Mongolian gerbil (Meriones unguiculatus), an important animal model in neurosciences, is presented. It combines high-quality histological material for identification of brain structures with reliable stereotaxic coordinates. The atlas consists of high-resolution images of frontal sections alternately stained for cell bodies (Nissl) and myelinated fibers (Gallyas) of 62 rostro-caudal levels at intervals of 350 μm. Brain structures were named according to the Paxinos nomenclature for rodents. The accuracy of the stereotaxic coordinate system was improved substantially by comparing and matching the series of histological sections to in vivo brain images of the gerbil obtained by magnetic resonance imaging (MRI). The skull outlines corresponding to the MR images were acquired using X-ray computerized tomography (CT) and were used to establish the relationship between coordinates of brain structures and skull. Landmarks such as lambda, bregma, ear canals and occipital crest can be used to line up skull and brain in standard atlas coordinates. An easily reproducible protocol allows sectioning of experimental brains in the standard frontal plane of the atlas.
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影响因子：
3
作者：
SCHULLER, G;RADTKESCHULLER, S;BETZ, M
通讯作者：
BETZ, M
影响因子：
4.8
作者：
Radtke-Schuller, Susanne;Seeler, Sabine;Grothe, Benedikt
通讯作者：
Grothe, Benedikt
影响因子：
2.5
作者：
Budinger, Eike;Brosch, Michael;Mylius, Judith
通讯作者：
Mylius, Judith
影响因子：
2.5
作者：
THIESSEN, DD;GOAR, S
通讯作者：
GOAR, S