Optimal pressure conditions for unbiased external ion accumulation in a two-dimensional radio-frequency quadrupole for Fourier transform ion cyclotron resonance mass spectrometry.
Optimal pressure conditions for unbiased external ion accumulation in a two-dimensional radio-frequency quadrupole for Fourier transform ion cyclotron resonance mass spectrometry.
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用于傅里叶变换离子回旋共振质谱的二维射频四极杆中无偏外部离子积累的最佳压力条件。
DOI：
10.1002/rcm.459
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Smith,RD
中科院分区：
文献类型：
--
作者：
Belov,ME;Gorshkov,MV;Alving,K;Smith,RD
当与在线分离相结合时（例如，毛细管液相色谱（LC）、傅立叶变换离子回旋共振质谱（FTICR-MS）为生物学应用，特别是蛋白质组学研究提供了有力的工具。FTICR-MS提供的灵敏度、动态范围和占空比已被证明通过位于FTICR单元外部的二维（2D）仅射频（rf）多极中的离子捕获和积累而增加。然而，重要的是在所需的m/z范围内检测离子而没有显著的偏差。在这项工作中，我们发现积累rf四极内的压力在获得“无偏”离子积累中起着重要作用。在脉冲和连续模式下进行压力优化。结果表明，在5 × 10− 4 ~ 5 × 10− 3 Torr的气压范围内，二维四极射频场可以实现无偏积累。 在最佳压力下进行的外部离子积累导致光谱采集速率和动态范围的增加。版权所有© 2001约翰威利父子有限公司。
When combined with on‐line separations (e.g., capillary liquid chromatography (LC)), Fourier transform ion cyclotron resonance mass spectrometry (FTICR‐MS) provides a powerful tool for biological applications, and particularly proteomic studies. The sensitivity, dynamic range, and duty cycle provided by FTICR‐MS have been shown to be increased by ion trapping and accumulation in a two‐dimensional (2D) radio‐frequency (rf)‐only multipole positioned externally to an FTICR cell. However, it is important that ions be detected across the desiredm/zrange without a significant bias. In this work we found that pressure inside the accumulation rf‐quadrupole plays an important role in obtaining ‘unbiased’ ion accumulation. Pressure optimization was performed in both pulsed and continuous modes. It was found that unbiased accumulation in a 2D rf‐only quadrupole could be achieved in the pressure range of 5 × 10−4to 5 × 10−3Torr. External ion accumulation performed at the optimal pressure resulted in an increase in both the spectrum acquisition rates and dynamic range. Copyright © 2001 John Wiley & Sons, Ltd.