Genotype Diversity and Distribution of Orientia tsutsugamushi Causing Scrub Typhus in Thailand
Genotype Diversity and Distribution of Orientia tsutsugamushi Causing Scrub Typhus in Thailand
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DOI：
10.1128/jcm.00355-11
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发表时间：
2011-07-01
影响因子：
9.4
通讯作者：
Gaywee, Jariyanart
中科院分区：
文献类型：
--
作者：
Ruang-areerate, Toon;Jeamwattanalert, Pimmada;Gaywee, Jariyanart
恙虫病是由恙虫病东方体分离株引起的一种广泛分布于亚太地区的螨传播疾病。关于免疫显性56 kDa型特异性抗原（TSA）基因的异质性的信息对于恙虫病特异性诊断检测和疫苗的设计和评价至关重要。采用间接免疫荧光法（IFA）和PCR方法，对O. 2004年至2007年期间，从泰国六个省的啮齿动物和不明原因发热患者中检测到恙虫病。确定了56 kDa TSA基因片段的序列，并评估了这些序列与先前确定的序列之间的关系。56-kDa TSA基因部分序列的系统发育分析表明O.泰国的恙虫病基因型。此外，遗传多样性分组的恙虫病剂分为两个常见的和五个罕见的基因型内六个省的泰国。O.本研究中描述的恙虫病与广泛用于设计和评价诊断测定和候选疫苗的原型菌株无关。因此，这些新的基因型应考虑未来的恙虫病检测和疫苗的开发。
Scrub typhus, caused by antigenically disparate isolates of Orientia tsutsugamushi, is a widely distributed mite-borne human disease in the Asia Pacific region. Information regarding the heterogeneity of the immunodominant 56-kDa type-specific antigen (TSA) gene is crucial for the design and evaluation of scrub typhus-specific diagnostic assays and vaccines. Using indirect immunofluorescence assays (IFA) and PCR assays, O. tsutsugamushi was detected samples from rodents and patients with fever of unknown origin obtained from six provinces of Thailand during 2004 to 2007. Sequences were determined for a fragment of the 56-kDa TSA gene, and the relationship between these sequences and those previously determined were assessed. The phylogenetic analyses of partial 56-kDa TSA gene sequences demonstrated wide diversity and distribution of O. tsutsugamushi genotypes in Thailand. Furthermore, the genetic diversity grouped the scrub typhus agents into two commonly and five infrequently found genotypes within six provinces of Thailand. The two most commonly found genotypes of O. tsutsugamushi described in this study do not associate with the prototype strains that are widely used for the design and evaluation of diagnostic assays and vaccine candidates. Thus, these new genotypes should be considered for future scrub typhus assay and vaccine development.