Activation of μ-opioid receptors differentially affects the preBotzinger Complex and neighbouring regions of the respiratory network in the adult rabbit
Activation of μ-opioid receptors differentially affects the preBotzinger Complex and neighbouring regions of the respiratory network in the adult rabbit
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DOI：
10.1016/j.resp.2020.103482
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发表时间：
2020-09-01
影响因子：
2.3
通讯作者：
Mutolo, Donatella
中科院分区：
文献类型：
--
作者：
Cinelli, Elenia;Bongianni, Fulvia;Mutolo, Donatella
呼吸网络的不同组分在阿片类药物诱导的呼吸抑制中的作用尚不清楚。在麻醉、切断迷走神经、麻痹和人工通气的成年家兔中，我们利用双侧微量注射（30-50 nl）μ-阿片受体激动剂[D-Ala（2），N-Me-Phe（4），Gly（5）-ol]-脑啡肽（DAMGO），研究了前Botzinger复合体（preBotC）和相邻Botzinger复合体（BotC）以及腹侧呼吸群吸气部分（iVRG）的贡献。观察到剂量依赖性效应。在preBotC和BotC中，0.1 mM DAMGO微量注射主要导致与紧张性膈活动和不规则（共济失调）呼吸模式相关的膈振幅峰值降低，这在preBotC中更为明显。在iVRG DAMGO引起的膈爆发在0.1 mM和呼吸暂停在0.5 mM的振幅和频率降低。局部5 mM纳洛酮逆转了呼吸暂停的影响。结果表明，呼吸网络的不同组成部分可能有助于阿片类药物引起的呼吸障碍。可提供上标/下标
The role of the different components of the respiratory network in the mediation of opioid-induced respiratory depression is still unclear. We investigated the contribution of the preBotzinger Complex (preBotC) and the neighbouring Botzinger Complex (BotC) and inspiratory portion of the ventral respiratory group (iVRG) in anesthetized, vagotomized, paralyzed and artificially ventilated adult rabbits making use of bilateral micro injections (30-50 nl) of the mu-opioid receptor agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin (DAMGO). Dose dependent effects were observed. In the preBotC and the BotC 0.1 mM DAMGO microinjections caused mainly reductions in peak phrenic amplitude associated with tonic phrenic activity and irregular (ataxic) patterns of breathing that were more pronounced in the preBotC. Apneic effects developed at 0.5 mM. In the iVRG DAMGO provoked decreases in amplitude and frequency of phrenic bursts at 0.1 mM and apnea at 0.5 mM. Local 5 mM naloxone reversed the apneic effects. The results imply that different components of the respiratory network may contribute to opioid-induced respiratory disorders. Superscript/Subscript Available