New approaches to single-cell analysis by capillary electrophoresis
New approaches to single-cell analysis by capillary electrophoresis
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DOI：
10.1016/s0165-9936(01)00051-6
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发表时间：
2001-09-01
影响因子：
13.1
通讯作者：
Lillard, SJ
中科院分区：
文献类型：
--
作者：
Zabzdyr, JL;Lillard, SJ
单个细胞的内容物可以很容易地通过毛细管电泳（CE）分离，并可以用多种方法检测。本文强调了CE单细胞测量的两个新兴趋势：(1)改进仪器以对单个细胞进行更有效的分析；(2)检测单个细胞中包含的不同类别的分析物。对以细胞为基础测量化学的兴趣日益增加，推动了样品处理和操作、自动化和检测领域仪器能力的发展，以改进分离和识别细胞内内容物。不同种类的细胞内分析物的检测（与早期工作中研究的相比）源于增强的检测器技术，衍生化化学知识的改进，以及对相关生物分析物的测量量和相应含义的新认识。本文主要介绍用于自动化单细胞分析和连续进样的仪器。本文还讨论了质谱法与CE联用对单个细胞分析物的检测和鉴定。最后，描述了从单细胞中检测肌醇1,4,5-三磷酸，RNA和黄体酮的新方法。(C) 2001 Elsevier Science B.V.版权所有
The contents of individual cells can be readily separated by capillary electrophoresis (CE) and detected with a variety of methods. This article emphasizes two emerging trends of single-cell measurements by CE: (1) refinement in instrumentation to perform more efficient analyses of individual cells and ( 2) detection of different classes of analytes contained in single cells. Increased interest in measuring chemistry on a cell by cell basis drives the development of instrumentation capabilities in the areas of sample handling and manipulation, automation, and detection for the improvement of fractionating and identifying intracellular contents. Detection of different classes of intracellular analytes (compared to those studied in the earlier work) has resulted from enhanced detector technology, improved knowledge of derivatization chemistry, and new perceptions about the measured amounts and corresponding meaning of relevant biological analytes. This article focuses on instrumentation for automated single-cell analysis and continuous cell injection. The coupling of mass spectrometry to CE for the detection and identification of analytes from individual cells is also discussed. Finally, novel methods for the detection of inositol 1,4,5-trisphosphate, RNA, and progesterone from single cells are described. (C) 2001 Elsevier Science B.V. All rights reserved.