Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage
Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage
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DOI：
10.1073/pnas.96.5.1921
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发表时间：
1999-03-02
影响因子：
11.1
通讯作者：
Haaf, T
中科院分区：
文献类型：
--
作者：
Raderschall, E;Golub, EI;Haaf, T
一种灵敏和快速的原位方法被开发出来，以可视化网站的单链（ss）DNA在培养细胞和实验测试动物。仅当取代的DNA为单链形式时，抗溴脱氧尿苷抗体才识别掺入染色体DNA中的卤代碱基类似物。处理后的细胞与DNA损伤剂或γ射线照射，ssDNA分子形成核灶中的剂量依赖性的方式在60分钟内。哺乳动物重组蛋白Rad51和复制蛋白A，然后积累在网站的ssDNA和形式的焦点，这表明这些网站的重组DNA修复。
A sensitive and rapid in situ method was developed to visualize sites of single-stranded (ss) DNA in cultured cells and in experimental test animals. Anti-bromodeoxyuridine antibody recognizes the halogenated base analog incorporated into chromosomal DNA only when substituted DNA is in the single strand form. After treatment of cells with DNA-damaging agents or gamma irradiation, ssDNA molecules form nuclear foci in a dose-dependent manner within 60 min. The mammalian recombination protein Rad51 and the replication protein A then accumulate at sites of ssDNA and form foci, suggesting that these are sites of recombinational DNA repair.