PHAGOCYTIC BEHAVIOR OF THE PREDATORY SLIME-MOLD, DICTYOSTELIUM-CAVEATUM - CELL NIBBLING
PHAGOCYTIC BEHAVIOR OF THE PREDATORY SLIME-MOLD, DICTYOSTELIUM-CAVEATUM - CELL NIBBLING
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DOI：
10.1016/s0014-4827(85)80006-9
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发表时间：
1985-01-01
影响因子：
3.7
通讯作者：
VOGEL, G
中科院分区：
文献类型：
--
作者：
WADDELL, DR;VOGEL, G
捕食性黏菌D. caveatum通过吞噬作用以其他变形虫为食。地方变形虫开始通过啃噬细胞碎片而以相同大小或更大的细胞为食。当以其他变形虫为食而不是细菌时，它们的体积会增加。这种行为类似于高等生物中的吞噬细胞。一种新的方法被用来跟踪吞噬的时间过程。一种裂解毒素，phallolysin，和突变体的毒素抗性被用于在一项测定中分离的吞噬细胞从猎物细胞。由于广谱细胞对毒素敏感，因此该方法具有普遍适用性。
The predatory slime mold, D. caveatum, feeds upon other amoebae by phagocytosis. The D. caveatum amoebae begin feeding upon cells the same size or larger by nibbling pieces of cells. While feeding upon other amoebae as opposed to bacteria, they increase in size. This behavior resembles that of phagocytes in higher organisms. A novel method was used to follow the time course of phagocytosis. A lytic toxin, phallolysin, and mutants resistant to the toxin were utilized in an assay to separate the phagocytes from the prey cells. Since a broad spectrum of cells are sensitive to the toxin, the method has general applicability.