Near-infrared spectroscopy as a complementary age grading and species identification tool for African malaria vectors
Near-infrared spectroscopy as a complementary age grading and species identification tool for African malaria vectors
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DOI：
10.1186/1756-3305-3-49
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发表时间：
2010-06-04
影响因子：
3.2
通讯作者：
Dowell, Floyd E.
中科院分区：
文献类型：
--
作者：
Sikulu, Maggy;Killeen, Gerry F.;Dowell, Floyd E.
近红外光谱技术（NIRS）是近年来应用于年龄分级和区分实验室饲养的狭义冈比亚按蚊和广义冈比亚按蚊复合体的阿拉伯按蚊的亲种。在这项研究中，我们进一步报告的准确性，同时估计年龄组和区分形态学上难以区分的An。冈比亚有限公司和一个.半场释放和野生种群的阿拉伯。9个不同日龄（1、3、5、7、9、11、12、14、16 d）的安氏按蚊（An. arabiensis和8个年龄段（1、3、5、7、9、10、11、12 d）的南方古猿（An.冈比亚有限公司饲养在半野外大笼系统内的250 × 60 × 40 cm笼中，gambiae s.l.，被纳入本研究。NIRS分类为雌性安氏按蚊。arabiensis和安.冈比亚有限公司在半野笼中饲养的7日龄AS的准确率分别为89%（n = 377）和78%（n = 327），并以89%（n = 704）的准确率区分它们。怀尔德抓住了安冈比亚公司以90%的准确度（n = 105）确定，而他们的预测年龄与解剖确定的生理年龄类别的预期平均实足年龄一致。这些发现对于监测控制方案具有重要意义，其中减少有能力传播疟疾的老年蚊子的比例是一个重要成果。
Near-infrared spectroscopy (NIRS) was recently applied to age-grade and differentiate laboratory reared Anopheles gambiae sensu strico and Anopheles arabiensis sibling species of Anopheles gambiae sensu lato complex. In this study, we report further on the accuracy of this tool for simultaneously estimating the age class and differentiating the morphologically indistinguishable An. gambiae s.s. and An. arabiensis from semi-field releases and wild populations. Nine different ages (1, 3, 5, 7, 9, 11, 12, 14, 16 d) of An. arabiensis and eight different ages (1, 3, 5, 7, 9, 10, 11, 12 d) of An. gambiae s.s. maintained in 250 x 60 x 40 cm cages within a semi-field large-cage system and 105 wild-caught female An. gambiae s.l., were included in this study. NIRS classified female An. arabiensis and An. gambiae s.s. maintained in semi-field cages as = 7 d old with 89% (n = 377) and 78% (n = 327) accuracy, respectively, and differentiated them with 89% (n = 704) accuracy. Wild caught An. gambiae s.l. were identified with 90% accuracy (n = 105) whereas their predicted ages were consistent with the expected mean chronological ages of the physiological age categories determined by dissections. These findings have importance for monitoring control programmes where reduction in the proportion of older mosquitoes that have the ability to transmit malaria is an important outcome.