The medicinal fungus Antrodia cinnamomea regulates DNA repair and enhances the radiosensitivity of human esophageal cancer cells.
The medicinal fungus Antrodia cinnamomea regulates DNA repair and enhances the radiosensitivity of human esophageal cancer cells.
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DOI：
10.2147/ott.s96355
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发表时间：
2016
影响因子：
4
通讯作者：
Chen YJ
中科院分区：
文献类型：
--
作者：
Liu YM;Liu YK;Wang LW;Huang YC;Huang PI;Tsai TH;Chen YJ
本研究探讨樟芝菌丝体发酵液（AC-MFB）对食道癌细胞放射敏感性的影响。本研究使用人CE 81 T/VGH鳞状和BE 3腺癌食管癌细胞。集落形成实验表明，AC-MFB预处理降低了放射性食管癌细胞的存活率，最大增敏率为1.91%，存活率为37%。DNA直方图研究表明，AC-MFB预处理增强了细胞周期停滞在G2/M期（最放射敏感的时期）。免疫荧光分析和Western印迹分析表明，AC-MFB延迟了γ-H2 AX的废除，上调了p21的表达，并减弱了辐射诱导的共济失调毛细血管扩张突变激酶和检查点激酶2的磷酸化。一项体内验证研究表明，AC-MFB处理与辐射对BALB/c小鼠中CE 81 T/VGH细胞的肿瘤生长延迟往往具有协同作用。提示该食用菌产品具有增强食管癌放疗效果的作用。
This study investigated the adjunctive effects of Antrodia cinnamomea mycelial fermentation broth (AC-MFB), a Taiwanese medicinal fungus, in enhancing the radiosensitivity of esophageal cancer cells. Human CE81T/VGH squamous and BE3 adenocarcinoma esophageal cancer cells were used in this study. A colony formation assay showed that pretreatment with AC-MFB decreased the survival of irradiated esophageal cancer cells, with a maximum sensitizer enhancement ratio of 1.91% and 37% survival. A DNA histogram study showed that AC-MFB pretreatment enhanced cell cycle arrest at the G2/M phase, the most radiosensitive phase. An immunofluorescence assay and a Western blotting assay showed that AC-MFB delayed the abrogation of γ-H2AX, upregulated p21 expression, and attenuated the radiation-induced phosphorylation of ataxia telangiectasia-mutated kinase and checkpoint kinase 2. An in vivo validation study showed that AC-MFB treatment tended to have a synergistic effect with radiation on the tumor growth delay of CE81T/VGH cells in BALB/c mice. These data suggest that this edible fungus product could enhance the effect of radiotherapy against esophageal cancer.