Basic fibroblast growth factor treatment delays age-related photoreceptor degeneration in Fischer 344 rats.
Basic fibroblast growth factor treatment delays age-related photoreceptor degeneration in Fischer 344 rats.
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碱性成纤维细胞生长因子治疗可延缓 Fischer 344 大鼠与年龄相关的光感受器变性。
DOI：
10.1006/exer.1996.0208
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发表时间：
1997
影响因子：
3.4
通讯作者：
J. Turner
中科院分区：
文献类型：
--
作者：
N. Lin;Wei;H. Sheedlo;J. Turner
本研究旨在探讨碱性成纤维细胞生长因子（bFGF）延迟Fischer 344大鼠视网膜感光细胞损失的潜力，该大鼠表现出与年龄相关的外周视网膜病变。8只16个月大的雄性Fischer 344大鼠右眼玻璃体内注射2.0微克bFGF，而左眼注射载体，注射后两个月处死所有大鼠。另外八只Fischer大鼠在16个月时注射了bFGF或溶剂，并在18个月时再次注射，然后在两个月后杀死。眼球摘除后，对眼睛进行光学显微镜、电子显微镜和形态测量分析。从锯齿缘到上级视网膜外核层（ONL）厚度为两个细胞的点的距离，称为死亡区，代表外周视网膜病变的程度，在bFGF和溶媒注射的Fischer大鼠中进行比较。这些测量结果显示，与接受载体注射的年龄匹配大鼠的视网膜中的死亡回退区相比，注射两次bFGF的眼睛的视网膜中的死亡回退区显著减少（P < 0.01）。然而，当与相应的注射载体的大鼠的视网膜相比时，在接受单一bFGF注射的18月龄大鼠的视网膜中的该变性区中没有显著差异（P > 0.05）。此外，注射bFGF的20月龄Fischer大鼠的上级视网膜中距锯齿缘2和3 mm处的ONL显著厚于溶剂对照大鼠视网膜中的相应区域。此外，接受两次bFGF注射的20月龄大鼠的上级视网膜中的光感受器细胞具有正常外观的内节和外节，而在Fisher大鼠的溶媒注射视网膜中，少数光感受器具有短的内节和外节。这些研究结果表明，注射bFGF到Fischer 344大鼠的眼睛中显着延迟感光细胞变性的进展，表明外源性bFGF可能作为这些老年视网膜中的生存促进因子。
This study was undertaken to investigate the potential of basic fibroblast growth factor (bFGF) to delay photoreceptor cell loss in retinas of Fischer 344 rats which exhibit an age-related peripheral retinopathy. Eight male 16-month-old Fischer 344 rats were injected intravitreally with 2.0 micrograms bFGF in the right eye, while the vehicle was injected into the left eye and all rats were killed at two months post-injection. Eight other Fischer rats were injected with either bFGF or vehicle at 16 and again at 18 months, then killed two months later. After enucleation, eyes were processed for light and electron microscopic and morphometric analyses. The distance from the ora serrata to the point where the outer nuclear layer (ONL) in the superior retina was two cells in thickness, called the die-back zone, that represents the extent of the peripheral retinopathy, was compared in bFGF- and vehicle-injected Fischer rats. These measurements revealed that the die-back zone in retinas of eyes injected twice with bFGF was significantly reduced (P < 0.01) when compared to this zone in retinas of age-matched rats that received vehicle injections. However, there was no significant difference (P > 0.05) in this degeneration zone in retinas of 18-month-old rats that received a single-bFGF injection when compared to retinas of respective vehicle-injected rats. In addition, the ONL at 2 and 3 mm from the ora serrata in the superior retina of bFGF-injected 20-month-old Fischer rats was significantly thicker than corresponding regions in retinas of vehicle-control rats. Furthermore, the photoreceptor cells in the superior retina of 20-month-old rats which received two injections of bFGF had normal-appearing inner and outer segments, while the few photoreceptors had short inner and outer segments in vehicle-injected retinas of Fisher rats. These findings reveal that injections of bFGF into eyes of Fischer 344 rats significantly delays the progress of photoreceptor cell degeneration suggesting that exogenous bFGF may act as a survival-promoting factor in these aged retinas.
培养的人视网膜色素上皮细胞表达碱性成纤维细胞生长因子及其受体。
影响因子：
2
作者：
Sternfeld,MD;Robertson,JE;Shipley,GD;Tsai,J;Rosenbaum,JT
通讯作者：
Rosenbaum,JT
正常和 RCS 大鼠成熟和发育视网膜中碱性成纤维细胞生长因子的免疫组织化学定位。
影响因子：
2
作者：
Connolly,SE;Hjelmeland,LM;LaVail,MM
通讯作者：
LaVail,MM
影响因子：
4.4
作者：
Kazuhiko Unoki;M. Lavail
通讯作者：
Kazuhiko Unoki;M. Lavail