Predicted functions and linkage Specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci
Predicted functions and linkage Specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci
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DOI：
10.1128/jb.00837-07
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发表时间：
2007-11-01
影响因子：
3.2
通讯作者：
Spratt, Brian G.
中科院分区：
文献类型：
--
作者：
Aanensen, David M.;Mavroldi, Angeliki;Spratt, Brian G.
最近已经确定了90种血清型肺炎链球菌的囊囊生物合成（CPS）基因座的序列。生物信息学被用于预测1,999个基因产物中1,973种的一般功能，并鉴定同一个同源性组，PFAM家族和Cazy Glycosylansylansferase族中的蛋白质。将CPS基因含量与54个已知囊多糖（CPS）结构相关联，提供了每个功能类别的不同同源组的特定功能的试验性分配（调节蛋白，合成CPS成分的酶，聚合酶，聚合酶，氟糖转移酶，果糖基蛋白酶基质酶，glycosymytransytransyprass，glycosyprossytransyprass，glycosymytransyprass酶，酶[GTS]，磷酸转移酶，乙酰转移酶和pyruvyltransferases）。由342 GTS（92个同源组）催化的糖苷链接的分配是有问题的，但是可以通过使用此大型CPS基因座和CPS结构来进行暂定分配，以将特定GTS的存在与特定的Glycosidic链接相关或通过比较具有非常相似CPS基因含量的血清型的CPS结构，将CPS重复单元与GTS的倒置或保留机制保持在CPS重复单位中。结构和基因含量之间的这些大规模比较分配了催化72％的GTS的链接，并且在32种具有已知重复单位结构的血清型中分配了所有链接。还鉴定出了非常相似的初始糖转移酶或糖基转移酶在不同血清型中催化不同链接的明确例子。这些分配应为生化研究提供刺激，以评估提出的反应。
The sequences of the capsular biosynthetic (cps) loci of 90 serotypes of Streptococcus pneumoniae have recently been determined. Bioinformatic procedures were used to predict the general functions of 1,973 of the 1,999 gene products and to identify proteins within the same homology group, Pfam family, and CAZy glycosyltransferase family. Correlating cps gene content with the 54 known capsular polysaccharide (CPS) structures provided tentative assignments of the specific functions of the different homology groups of each functional class (regulatory proteins, enzymes for synthesis of CPS constituents, polymerases, flippases, initial sugar transferases, glycosyltransferases [GTs], phosphotransferases, acetyltransferases, and pyruvyltransferases). Assignment of the glycosidic linkages catalyzed by the 342 GTs (92 homology groups) is problematic, but tentative assignments could be made by using this large set of cps loci and CPS structures to correlate the presence of particular GTs with specific glycosidic linkages, by correlating inverting or retaining linkages in CPS repeat units with the inverting or retaining mechanisms of the GTs predicted from their CAZy family membership, and by comparing the CPS structures of serotypes that have very similar cps gene contents. These large-scale comparisons between structure and gene content assigned the linkages catalyzed by 72% of the GTs, and all linkages were assigned in 32 of the serotypes with known repeat unit structures. Clear examples where very similar initial sugar transferases or glycosyltransferases catalyze different linkages in different serotypes were also identified. These assignments should provide a stimulus for biochemical studies to evaluate the reactions that are proposed.