The JAK/STAT signaling pathway is required for the initial choice of sexual identity in Drosophila melanogaster
The JAK/STAT signaling pathway is required for the initial choice of sexual identity in Drosophila melanogaster
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DOI：
10.1016/s1097-2765(00)80451-7
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发表时间：
2000-03-01
期刊：
影响因子：
16
通讯作者：
Schedl, P
中科院分区：
文献类型：
--
作者：
Jinks, TM;Polydorides, AD;Schedl, P
果蝇性别认同的选择是由测量 X 染色体与常染色体比率 (X/A) 的系统决定的。该系统依赖于 1X 和 2X 细胞核中 X 连锁分子基因的不相等表达。分子在 2X/2A 细胞核中激活特殊的 Sri 启动子 Sxl-Pe，但在 1X/2A 细胞核中则不然。通过多聚化保守的 Sxl-Pe 序列块，我们生成了功能获得启动子 Sxl-Pe(GOF)，它在 1X/2A 细胞核中具有不适当的活性。 GOF 活性需要 X 连锁未配对 (upd) 基因，该基因编码果蝇 JAK/STAT 信号通路的配体。 opd 还充当调节野生型 Sxl-Pe 报告基因的分子元件。我们证明 JAK 激酶 Hopscotch 和 STAT DNA 结合蛋白 Marelle 也是 Sxl-Pe 激活所必需的。
The choice of sexual identity in Drosophila is determined by a system that measures the X chromosome to autosome ratio (X/A). This system depends upon unequal expression of X-linked numerator genes in 1X and 2X nuclei. The numerators activate a special Sri promoter, Sxl-Pe, in 2X/2A nuclei, but not 1X/2A nuclei. By multimerizing a conserved Sxl-Pe sequence block, we generated a gain-of-function promoter, Sxl-Pe(GOF), that is inappropriately active in 1X/2A nuclei. GOF activity requires the X-linked unpaired (upd) gene, which encodes a ligand for the Drosophila JAK/STAT signaling pathway. opd also functions as a numerator element in regulating wild-type Sxl-Pe reporters. We demonstrate that the JAK kinase, Hopscotch, and the STAT DNA-binding protein, Marelle, are also required for Sxl-Pe activation.