miR-143 is critical for the formation of primordial follicles in mice.
miR-143 is critical for the formation of primordial follicles in mice.
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DOI：
10.2741/4122
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Jianfang Zhang;Xiaowen Ji;D. Zhou;Yueqin Li;Junkai Lin;Jiali Liu;Hao-shu Luo;S. Cui
中科院分区：
文献类型：
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作者：
Jianfang Zhang;Xiaowen Ji;D. Zhou;Yueqin Li;Junkai Lin;Jiali Liu;Hao-shu Luo;S. Cui
虽然microRNAs（miRNAs）参与了基因网络的微调，但mmu-mir-143（miR-143）在哺乳动物卵巢发育中的作用尚未在体外研究。我们研究了miR-143在小鼠卵巢原始卵泡形成过程中的表达和功能。实时聚合酶链反应分析显示，从性交后15.5天到产后4天，miR-143的表达在原始卵泡形成期间增加。原位杂交检测miR-143定位于前颗粒细胞。为了研究miR-143在原始卵泡形成中的功能，我们建立了体外电穿孔转染模型，使miR-143在培养的卵巢中有效地上调和抑制表达。进一步的研究表明miR-143通过抑制前颗粒细胞增殖和下调细胞周期相关基因的表达来抑制原始卵泡的形成。这些发现表明，miR-143对原始卵泡的形成至关重要，并调节卵巢发育和功能。
Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of mmu-mir-143 (miR-143) in mammalian ovary development have not been studied in vitro. We investigated the expression and function of miR-143 in the mouse ovary during primordial follicle formation. Real-time polymerase chain reaction analysis showed that miR-143 expression increased during primordial follicle formation from 15.5 days post-coitus to 4 days post-partum. miR-143 was located in pregranulosa cells by in situ hybridization. To study the function of miR-143 in primordial follicle formation we established an electroporation transfection model in vitro that allowed miR-143 expression to be efficiently upregulated and inhibited in cultured ovaries. Further studies showed that miR-143 inhibited the formation of primordial follicles by suppressing pregranulosa cell proliferation and downregulating the expression of genes related to the cell cycle. These findings suggest that miR-143 is critical for the formation of primordial follicles and regulates ovarian development and function.