An intramolecular charge transfer process based fluorescent probe for monitoring subtle pH fluctuation in living cells.
An intramolecular charge transfer process based fluorescent probe for monitoring subtle pH fluctuation in living cells.
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DOI：
10.1016/j.talanta.2016.10.012
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Mingtai Sun;L. Du;Huan Yu;Kui Zhang;Yang Liu;Suhua Wang
中科院分区：
文献类型：
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作者：
Mingtai Sun;L. Du;Huan Yu;Kui Zhang;Yang Liu;Suhua Wang
细胞内pH值及其波动的监测是至关重要的，因为细胞的细胞器具有不同的pH分布。本文构建了一种新的小分子荧光探针HBT-O，用于监测活细胞中中性至酸性范围内的细微pH值。该探针表现出良好的水溶性，响应于pH值的显著的绿松石到橄榄石发射颜色变化，和巨大的荧光深色位移为1718 cm-1，以及当pH值从中性变为酸性时荧光强度增加。因此，探针HBT-O可以区分从中性到酸性的正常pH值范围内的细微变化，具有显著的荧光变化。这些性质可以归因于探针在不同pH值的缓冲溶液中质子化后的分子内电荷转移（ICT）过程。此外，该探针是可逆的，对活细胞几乎无毒。利用该探针检测活细胞中pH值的变化，显示出不同的荧光颜色和强度。这些发现表明，该探针将在生物学和生物医学研究中找到有用的应用。
It is crucial to monitor intracellular pH values and their fluctuation since the organelles of cells have different pH distribution. Herein we construct a new small molecule fluorescent probe HBT-O for monitoring the subtle pH values within the scope of neutral to acid in living cells. The probe exhibited good water solubility, a marked turquoise to olivine emission color change in response to pH, and tremendous fluorescence hypochromatic shift of ∼50 nm (1718 cm−1) as well as the increased fluorescence intensity when the pH value changed from neutral to acid. Thus, the probe HBT-O can distinguish the subtle changes in the range of normal pH values from neutral to acid with significant fluorescence changes. These properties can be attributed to the intramolecular charge transfer (ICT) process of the probe upon protonation in buffer solutions at varied pH values. Moreover, the probe was reversible and nearly non-toxic for living cells. Then the probe was successfully used to detect pH fluctuation in living cells by exhibiting different fluorescence colors and intensity. These findings demonstrate that the probe will find useful applications in biology and biomedical research.