Regulatory mechanisms of oxidative species and phytohormones in marine microalgae Isochrysis zhangjiangensis under nitrogen deficiency
Regulatory mechanisms of oxidative species and phytohormones in marine microalgae Isochrysis zhangjiangensis under nitrogen deficiency
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DOI：
10.1016/j.algal.2016.05.025
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发表时间：
2016-07
影响因子：
5.1
通讯作者：
Shuang Wu;Yingying Meng;Xupeng Cao;Song Xue
中科院分区：
文献类型：
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作者：
Shuang Wu;Yingying Meng;Xupeng Cao;Song Xue
氧化物质和植物激素是植物调控胁迫反应的重要因子。本研究研究了缺氮条件下异菊张江微藻4种氧化物质O2−、H2O2、ClO−和NO的含量变化，以及植物激素水杨酸（SA）、茉莉酸（JA）和叶酸（FA）的含量变化。O2−和h2o2含量与微藻光合活性呈正相关，而NO和ClO−含量与微藻光合活性呈相反关系。在高等植物中，存在NO和ROS相互作用的平衡模型，揭示了NO和h2o2的协同调节病原体诱导的超敏反应相关的细胞死亡。因为在充满氮和缺乏氮的di中，NO和h2o2的水平。张江藻不同时高，我们提出作为单细胞低等植物的微藻。张江氏遵循这一模式，调控细胞内不同类型的氧化物种，尤其是从充氮状态培养到相应的缺氮状态，避免细胞死亡。结合氮胁迫下微藻中SA、JA和FA含量的相应变化，我们提出了氧化物种相互作用的平衡模式，以及氮同化、光合作用和SA、JA、FA的生物合成之间的相关性可能最初存在于藻类中，植物在从藻类到高等植物的进化过程中一直遵循这一模式并保持这种相关性，以保护自己免受胁迫条件的影响。
Oxidative species and phytohormones are important factors in plants that can regulate stress responses. In this study, the content variations of four oxidative species, O2−, H2O2, ClO−, and NO, and the phytohormones, salicylic acid (SA), jasmonic acid (JA), and folic acid (FA), were investigated in the microalgae Isochrysiszhangjiangensisunder nitrogen deficiency. The O2−and H2O2contents were found to be positively correlated with the photosynthetic activity of the microalgae, while the opposite relationship was observed for NO and ClO−. In higher plants, there is a balance model for NO and ROS interactions, which revealed that the cooperation of NO and H2O2regulates the pathogen-induced hypersensitive response-associated cell death. Because the NO and H2O2levels in both nitrogen-replete and nitrogen-depletedI. zhangjiangensiswere not simultaneously high, we proposed that as a unicellular lower plant, the microalgaeI. zhangjiangensisfollows this model and regulates the different types of intracellular oxidative species, especially when they were cultured from nitrogen sufficient condition to corresponding nitrogen deficient condition, to avoid cell death. Together with the corresponding changes in the SA, JA and FA contents in the nitrogen-stressed microalgae, we proposed that the balance model of oxidative species interactions, as well as the correlations among nitrogen assimilation, photosynthesis and the biosyntheses of SA, JA, and FA, may originally exist in algae, and plants have followed this model and maintained these correlations all along to protect themselves from stressful conditions during their evolutions from alga to higher plants.