Genome-wide identification and classification of the Hsf and sHsp gene families in Prunus mume, and transcriptional analysis under heat stress
Genome-wide identification and classification of the Hsf and sHsp gene families in Prunus mume, and transcriptional analysis under heat stress
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DOI：
10.7717/peerj.7312
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发表时间：
2019-07
期刊：
影响因子：
2.7
通讯作者：
X. Wan;Jie Yang;Cong Guo;M. Bao;Junwei Zhang
中科院分区：
文献类型：
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作者：
X. Wan;Jie Yang;Cong Guo;M. Bao;Junwei Zhang
热激转录因子（Hsfs）对热激蛋白（Hsps）的转录激活在植物热胁迫反应中起着重要作用。梅花是一种特色鲜明的观赏木本植物，品种丰富，花色多样。在本研究中，在梅中鉴定出18个Hsfs和24个小Hsps（sHsps）。分析了它们的染色体定位、蛋白质结构域、保守基序、系统发育关系和外显子-内含子结构，并与拟南芥Hsfs或sHsps进行了比较。将18个PmHsf成员分为A、B和C三类。共24个PmsHsps分为8个亚家族（CI至CIII，P，内质网，M和CI或P相关）。定量逆转录PCR分析表明，A2，A7和A9组的成员成为突出的热休克后，表明他们参与了一个关键的调节作用的耐热性。大多数PmsHsp基因在暴露于HS后上调。总体而言，我们的数据有助于提高对梅Hsf和sHsp基因家族复杂性的理解，并为指导未来系统研究调查Hsf和sHsp基因家族的作用提供了基础。
The transcriptional activation of heat shock proteins (Hsps) by heat shock transcription factors (Hsfs) is presumed to have a pivotal role in plant heat stress (HS) response. Prunus mume is an ornamental woody plant with distinctive features, including rich varieties and colors. In this study, 18 Hsfs and 24 small Hsps (sHsps) were identified in P. mume. Their chromosomal locations, protein domains, conserved motifs, phylogenetic relationships, and exon–intron structures were analyzed and compared with Arabidopsis thaliana Hsfs or sHsps. A total of 18 PmHsf members were classified into three major classes, A, B, and C. A total of 24 PmsHsps were grouped into eight subfamilies (CI to CIII, P, endoplasmic reticulum, M, and CI- or P-related). Quantitative reverse transcription PCR analysis revealed that members of the A2, A7, and A9 groups became the prominent Hsfs after heat shock, suggesting their involvement in a key regulatory role of heat tolerance. Most of the PmsHsp genes were up-regulated upon exposure to HS. Overall, our data contribute to an improved understanding of the complexity of the P. mume Hsf and sHsp gene families, and provide a basis for directing future systematic studies investigating the roles of the Hsf and sHsp gene families.