ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize.
ZmCPK11 is involved in abscisic acid-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signalling in maize.
复制标题
ZmCPK11 参与脱落酸诱导的抗氧化防御，并在玉米脱落酸信号传导中发挥 ZmMPK5 上游的作用
DOI：
10.1093/jxb/ers366
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发表时间：
2013-02
影响因子：
6.9
通讯作者：
Jiang M
中科院分区：
文献类型：
--
作者：
Ding Y;Cao J;Ni L;Zhu Y;Zhang A;Tan M;Jiang M
钙依赖性蛋白激酶（Ca 2 +-dependent protein kinases，CDPKs）参与脱落酸（abscisic acid，阿坝）介导的种子萌发、萌发后生长、气孔运动和植物抗逆性等生理过程。然而，目前尚不清楚CDPKs是否参与ABA诱导的抗氧化防御。在本研究中，玉米CDPK ZmCPK 11在ABA诱导的抗氧化防御中的作用以及ZmCPK 11和ZmMPK 5（玉米ABA激活的丝裂原激活蛋白激酶（MAPK））在阿坝信号传导中的关系进行了研究。阿坝和H2 O2处理诱导ZmCPK 11的表达和提高ZmCPK 11的活性，而ABA诱导的ZmCPK 11的表达和活性的提高需要H2 O2。瞬时基因表达分析和原生质体瞬时RNA干扰（RNAi）试验表明，ZmCPK 11参与ABA诱导的超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）的表达和活性上调，并参与H2 O2的产生。此外，ZmCPK 11被证明是必需的表达和活性的ZmMPK 5的阿坝信号的上调，但ZmMPK 5有ABA诱导的表达和活性的ZmCPK 11的上调的影响很小。此外，瞬时基因表达分析结合瞬时RNAi试验表明，ZmCPK 11的ZmMPK 5上游的抗氧化酶的活性调节。这些结果表明，ZmCPK 11参与ABA诱导的抗氧化防御和ZmMPK 5的阿坝信号转导在玉米中的上游功能。
Calcium-dependent protein kinases (CDPKs) have been shown to be involved in abscisic acid (ABA)-mediated physiological processes, including seed germination, post-germination growth, stomatal movement, and plant stress tolerance. However, it is not clear whether CDPKs are involved in ABA-induced antioxidant defence. In the present study, the role of the maize CDPK ZmCPK11 in ABA-induced antioxidant defence and the relationship between ZmCPK11 and ZmMPK5, a maize ABA-activated mitogen-activated protein kinase (MAPK), in ABA signalling were investigated. Treatments with ABA and H2O2 induced the expression of ZmCPK11 and increased the activity of ZmCPK11, while H2O2 was required for the ABA-induced increases in the expression and the activity of ZmCPK11. The transient gene expression analysis and the transient RNA interference (RNAi) test in protoplasts showed that ZmCPK11 is involved in ABA-induced up-regulation of the expression and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), and in the production of H2O2. Further, ZmCPK11 was shown to be required for the up-regulation of the expression and the activity of ZmMPK5 in ABA signalling, but ZmMPK5 had very little effect on the ABA-induced up-regulation of the expression and the activity of ZmCPK11. Moreover, the transient gene expression analysis in combination with the transient RNAi test in protoplasts showed that ZmCPK11 acts upstream of ZmMPK5 to regulate the activities of antioxidant enzymes. These results indicate that ZmCPK11 is involved in ABA-induced antioxidant defence and functions upstream of ZmMPK5 in ABA signalling in maize.
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DOI：
10.1073/pnas.0907205106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Jammes, Fabien;Song, Charlotte;Kwak, June M.
通讯作者：
Kwak, June M.
影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
4.9
作者：
Chen, Songbiao;Tao, Lizen;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang
影响因子：
6.9
作者：
Jiang, MY;Zhang, JH
通讯作者：
Zhang, JH