Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate
Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate
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黑海鞘血细胞裂解液中钒的凝胶过滤分析
DOI：
10.1002/jcp.1040930217
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发表时间：
1977
影响因子：
5.6
通讯作者：
Guy C. McLeod
中科院分区：
文献类型：
--
作者：
K. Gilbert;K. Kustin;Guy C. McLeod
通过原子吸收光谱分析来自匀浆黑海鞘血细胞裂解物的交联凝胶过滤的级分中的钒。结果不受 4-21°C 温度和 0.09-1.0 M (NaCl) 范围内离子强度的影响。 pH 值高于 2.2 时，上清液中钒明显损失。 pH 2.1、厌氧条件下的实验表明，绿色色原和含钒条带分别洗脱。在这些实验条件下，含钒物质的分子量相对较低（⋍1,000）。
Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were unaffected by temperature from 4–21°C, and by ionic strength in the range 0.09‐1.0 M (NaCl). Appreciable loss of vanadium in the supernatant was experienced above pH 2.2. Experiments at pH 2.1 under anaerobic conditions show that the green chromogen and the vanadium‐containing bands elute separately. Under these experimental conditions, the vanadium‐containing species is of relatively low (⋍ 1,000) molecular weight.