Overexpression of PdC3H17 Confers Tolerance to Drought Stress Depending on Its CCCH Domain in Populus
Overexpression of PdC3H17 Confers Tolerance to Drought Stress Depending on Its CCCH Domain in Populus
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PdC3H17 的过表达赋予杨树对干旱胁迫的耐受性，这取决于其 CCCH 结构域
DOI：
10.3389/fpls.2019.01748
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Guohua Chai
中科院分区：
文献类型：
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作者：
Yamei Zhuang;Yang Zhang;Sihui Chen;Dian Wang;Qing Liu;Gongke Zhou;Guohua Chai
植物CCCH锌指蛋白主要在转录后水平控制生长、发育和逆境反应。目前，有关植物CCCH蛋白在抗旱性中的作用的报道有限。在本研究中，我们提供的证据表明，美洲黑杨PdC3H17与耐旱性和反应有关。与对照相比，过表达PdC3H17导致杨树矮化，茎水势升高，表现出更强的光合作用和清除ROS的能力，从而增强了对干旱胁迫的抗性。因此，干旱处理后，这些高表达系的茎伸长和增粗速率均高于对照。然而，PdC3H17的CCCH结构域以外的编码区的过表达大致表现出类似WT的生理和抗旱表型，这表明控制这些过程的需要CCCH结构域。此外，PdC3H17的N末端序列在酵母细胞中具有转录活性。综上所述，我们的结果表明，PdC3H17可能依赖于其CCCH结构域来控制杨树的耐旱性。
Plant CCCH zinc finger proteins control growth, development, and stress responses mainly at the post-transcriptional level. Currently, limited reports are available about the roles of plant CCCH proteins in drought tolerance. In this study, we provided evidence showing that PdC3H17 from Populus deltoides × P. euramericana involves drought tolerance and response. Overexpression of PdC3H17 in poplar caused dwarf, resulted in higher stem water potential, and showed increased photosynthetic and ROS-scavenging abilities, thereby enhancing tolerance to drought stress, compared to controls. Accordingly, after drought treatment the stem elongation and thickening rates of these overexpression lines were higher than those of the controls. However, overexpression of the coding region excluding the CCCH domain of PdC3H17 roughly exhibited WT-like physiological and drought-resistant phenotypes, indicating the requirement of the CCCH domain for PdC3H17 controlling these processes. In addition, N-terminal sequence of PdC3H17 was found to possess transcriptional activity ability in yeast cells. Together, our results suggest that PdC3H17 may depend on its CCCH domain to control drought tolerance in Populus.