Evaluation of FT-IR and Nile Red methods for microalgal lipid characterization and biomass composition determination
Evaluation of FT-IR and Nile Red methods for microalgal lipid characterization and biomass composition determination
复制标题
FT-IR 和尼罗红方法对微藻脂质表征和生物质成分测定的评估
DOI：
10.1016/j.biortech.2012.09.123
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发表时间：
2013-01-01
影响因子：
11.4
通讯作者：
Chen, Huan-Lin
中科院分区：
文献类型：
--
作者：
Feng, Guo-Dong;Zhang, Fang;Chen, Huan-Lin
为了快速准确地表征微藻细胞的脂质含量，对6种典型的真核和原核藻类进行了重量测定、FT-IR和尼罗红（NR）染色研究。FT-IR和Nile Red是相对定量方法，与重量法相比，需要标准曲线。FT-IR方法同时测定了脂质、碳水化合物和蛋白质的含量，假设藻类细胞仅由这三种成分组成。尼罗红法是一种比较快速的荧光光谱法中性脂含量表征方法，可以通过荧光显微镜定位藻细胞的脂体。根据样品来源和处理目的，重量测定法适用于低频监测的大规模培养，FT-IR和Nile Red适用于中频监测的一般实验室培养，特别是Nile Red适用于需要高频筛选的小样本。(C) 2012 Elsevier Ltd.版权所有。
To characterize lipid content of microalgal cells rapidly and accurately, the gravimetric determination, FT-IR and Nile Red (NR) staining were investigated on six typical eukaryotic and prokaryotic algae species. FT-IR and Nile Red were relative quantification methods and a standard curve was required in contrast to the gravimetric method. The FT-IR method determined the lipid, carbohydrate and protein contents simultaneously assuming that the algal cells only consisted of those three components. The Nile Red method was a relatively rapid method for neutral lipid content characterization by spectrofluorometry and could locate lipid body of the algal cell by fluorescence microscopy. According to sample sources and processing purposes, the gravimetric determination was preferable for large-scale cultivation with low-frequency monitoring, while FT-IR and Nile Red were suitable for general laboratory cultivation with medium-frequency monitoring, in particularly Nile Red was appropriate for small samples when high-frequency screening was required. (C) 2012 Elsevier Ltd. All rights reserved.