A NLRC3-like gene from blunt snout bream (Megalobrama amblycephala): Molecular characterization, expression and association with resistance to Aeromonas hydrophila infection
A NLRC3-like gene from blunt snout bream (Megalobrama amblycephala): Molecular characterization, expression and association with resistance to Aeromonas hydrophila infection
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来自钝口鳊（团头鲂）的 NLRC3 样基因：分子特征、表达及其与嗜水气单胞菌感染抗性的关联
DOI：
10.1016/j.fsi.2017.02.018
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发表时间：
2017
影响因子：
4.7
通讯作者：
Hong Liu
中科院分区：
文献类型：
--
作者：
Fengjuan Zhou;Qifeng Zhan;Zhujin Ding;Lina Su;Jun Fan;Lei Cui;Nan Chen;Weimin Wang;Hong Liu
NLRC (the nucleotide-oligomerization domain (NOD)-like receptor subfamily C) consists of teleost-specific NLRs (NOD-like receptors) and plays pivotal roles in microbial recognition and regulation of innate immune response. In this study, we cloned and characterized aNLRC3-likegene (MamNLRC3-like) from blunt snout bream (Megalobrama amblycephala) by using the quantitative real-time PCR method, and analyzed the correlation between its polymorphisms and resistance toAeromonas hydrophilainfection. The full length cDNA ofMamNLRC3-likewas 2863 bp, with a 5′-UTR of 169 bp, ORF of 2301 bp and 3′-UTR of 393 bp, encoding 766 amino acid residues. MamNLRC3-like consisted of a conserved NACHT domain, a fish-specific NACHT associated domain (FISNA) and a PYRIN effective domain. During early developmental stages,MamNLRC3-likewas most highly expressed at 39.4 hpf (hours post fertilization, hatching stage) and constitutively detected in various healthy tissues. The expression ofMamNLRC3-likewas strongly induced byA. hydrophilainfection and peaked in the head kidney, liver, intestine, and trunk kidney at 12 h post infection. Six SNPs (single nucleotide polymorphisms) fromMamNLRC3-like, with 2 in introns and 4 in exons, were identified by direct sequencing, in which 6515C/T was a novel non-synonymous mutation. HRM (high resolution melting) genotyping of the 6 loci showed that locus 6515C/T SNP was associated with the resistance/susceptibility of blunt snout bream toA. hydrophilainfection (P< 0.01). The CC genotype fish were more resistant than CT carriers (P< 0.01). This study suggests that theMamNLRC3-likegene might play an important role in the innate immunity of blunt snout bream and could be used as a candidate marker for genetic selection ofA. hydrophila-resistant blunt snout bream.
