Investigation of a Salmonellosis Outbreak Caused by Multidrug Resistant Salmonella Typhimurium in China
Investigation of a Salmonellosis Outbreak Caused by Multidrug Resistant Salmonella Typhimurium in China
复制标题
我国一起多重耐药鼠伤寒沙门氏菌引起的沙门氏菌疫情调查
DOI：
10.3389/fmicb.2020.00801
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发表时间：
2020-04-29
影响因子：
5.2
通讯作者：
Sun, Yansong
中科院分区：
文献类型：
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作者：
Xiang, Ying;Li, Fuxiang;Sun, Yansong
多药耐药沙门氏菌的迅速出现是一个全球性的公共卫生问题，因为近年来的疫情大多是由多药耐药菌株引起的。在此，我们评估了由多重耐药鼠伤寒沙门氏菌（S.鼠伤寒沙门氏菌）通过采用流行病学和实验室调查，使用常规方法和全基因组测序（WGS）。参加宴会的12人中有11人出现胃肠道症状，8人出现S。回收鼠伤寒菌株。分离的暴发菌株表现出多重耐药（MDR），并降低了对环丙沙星的敏感性，环丙沙星是WHO推荐的临床治疗肠道感染的一线药物。耐药性（AMR）基因分析表明，这些暴发菌株的MDR表型可能是由于存在大量AMR基因，包括blaOXA-1和blaTEM-1 β-内酰胺酶基因，这些基因通常是质粒携带的，易于转移。进一步的毒力基因分析表明，这些暴发菌株还携带有大量的毒力基因，包括2种沙门氏菌致病岛（SPI-1和SPI-2）和许多与粘附相关的毒力基因。基于脉冲场凝胶电泳数据的聚类分析和基于WGS的系统发育分析表明，爆发克隆密切相关，因此可能来自当地菌株。这次由多药耐药鼠伤寒沙门氏菌引起的疫情突出了政府改进沙门氏菌感染预防和控制策略的必要性。
The rapid emergence of multidrug resistantSalmonellais a global public-health concern as outbreaks in recent years have mostly been caused by multidrug resistant strains. Here, we evaluated an outbreak in China caused by multidrug resistantSalmonella entericaserovar Typhimurium (S. Typhimurium) by employing an epidemiological and laboratory investigation using conventional methods and whole genome sequencing (WGS). Eleven of the 12 people who participated in a banquet showed gastrointestinal symptoms, and 8S. Typhimurium strains were recovered. Isolated outbreak strains showed multidrug resistance (MDR), and decreased susceptibility to ciprofloxacin, a first-line drug recommended by WHO for clinical treatment of intestinal infections. Antimicrobial resistance (AMR) gene analysis indicated that the MDR phenotype of these outbreak strains may be due to the presence of a number of AMR genes, including theblaOXA-1 andblaTEM-1 beta-lactamase genes, which are often plasmid-borne and easily transferred. Further virulence gene analysis indicated that these outbreak strains also carried a large number of virulence genes, including 2 types ofSalmonellapathogenicity islands (SPI-1 and SPI-2) and many adhesion-related virulence genes. Cluster analysis based on pulse-field gel electrophoresis data and phylogenetic analysis based on WGS revealed that the outbreak clone was closely related to and thus probably derived from local strains. This outbreak caused by multidrug resistantS.Typhimurium highlights the need for government improved strategies for the prevention and control ofSalmonellainfections.