Leukemia inhibitory factor-expressing human embryonic lung fibroblasts as feeder cells for human embryonic germ cells
Leukemia inhibitory factor-expressing human embryonic lung fibroblasts as feeder cells for human embryonic germ cells
复制标题
表达白血病抑制因子的人胚胎肺成纤维细胞作为人胚胎生殖细胞的饲养细胞
DOI：
10.1159/000107637
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Wang, Yaping
中科院分区：
文献类型：
--
作者：
Li, Fangfei;Liu, Yonggang;Wang, Yaping
一个强大的培养系统是维持人类胚胎生殖细胞（hEG）的增殖和发育潜力的关键。在这里，我们使用过表达白血病抑制因子（LIF）的人胚肺成纤维细胞（hELF）作为饲养细胞，以支持hEG细胞的自我更新。我们研究的形态，基因表达和发育潜力的hEG细胞生长在饲养层的LIF表达hELF（hELF/LIF）细胞。hEG细胞对碱性磷酸酶（AP）、阶段特异性胚胎抗原（SSEA）-1、SSEA- 4、肿瘤排斥抗原（TRA）-1-60和TRA- 1 - 81呈阳性。此外，维持在hELF/lif上的hEG细胞表达更高水平的多能性基因，如Oct 4和Nanog。此外，维持在hELF/lif细胞上的hEG细胞在作为胚状体生长时产生分化的组织，这与起始群体的广泛发育潜力一致。我们的研究结果表明，hELF/LIF饲养层可以支持hEG细胞的增殖，LIF信号在这个过程中起着至关重要的作用。这种人源性培养系统为临床应用中更常用的小鼠源性饲养层提供了一种有吸引力的替代方案。版权所有（C）2007 S. Karger AG，巴塞尔
A robust culture system is critical for maintaining both proliferation and the developmental potential of human embryonic germ ( hEG) cells. Here, we use human embryonic lung fibroblasts ( hELF) overexpressing leukemia inhibitory factor (LIF) as feeder cells to support the self-renewal of hEG cells. We examine the morphology, gene expression, and developmental potential of hEG cells grown on a feeder layer of LIF-expressing hELF (hELF/lif) cells. hEG cells were positive for alkaline phosphatase (AP), stage-specific embryonic antigen (SSEA)-1, SSEA- 4, tumor rejection antigen (TRA)-1-60, and TRA- 1 - 81. In addition, hEG cells maintained on hELF/lif expressed higher levels of pluripotency genes such as Oct4 and Nanog. In addition, hEG cells maintained on hELF/lif cells gave rise to differentiated tissues when grown as embryoid bodies, consistent with the broad developmental potential of the starting population. Our results suggest that a hELF/lif feeder layer can support the proliferation of hEG cells, and that LIF signaling plays an essential role in this process. This human-derived culture system provides an attractive alternative to more commonly used mouse-derived feeder layers for use in clinical applications. Copyright (C) 2007 S. Karger AG, Basel