Quercetin Protects Yeast Saccharomyces cerevisiae pep4 Mutant from Oxidative and Apoptotic Stress and Extends Chronological Lifespan
Quercetin Protects Yeast Saccharomyces cerevisiae pep4 Mutant from Oxidative and Apoptotic Stress and Extends Chronological Lifespan
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DOI：
10.1007/s00284-017-1412-x
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发表时间：
2018-05-01
影响因子：
2.6
通讯作者：
Dyavaiah, Madhu
中科院分区：
文献类型：
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作者：
Alugoju, Phaniendra;Janardhanshetty, Sudharshan Setra;Dyavaiah, Madhu
酵母酿酒酵母PEP 4基因编码空泡内肽酶蛋白酶A（Pep4p），其是编码组织蛋白酶D的人CTSD基因的同源物。人类CTSD基因突变可导致多种神经退行性疾病。在这项研究中，我们已经表明，酵母pep4突变体细胞是高度敏感的氧化和凋亡应激诱导的过氧化氢和乙酸，分别。pep4a匕首细胞还显示出活性氧簇（ROS）、凋亡标记物的积累以及时间寿命的缩短。与此相反，槲皮素预处理保护pep4突变体的氧化和凋亡应激诱导的敏感性，清除活性氧和减少凋亡标志物。槲皮素处理的pep4a匕首细胞的百分比活力更显着，并增加对氧化，凋亡和热应激的应激抵抗力在时序老化。从我们的实验结果中，我们得出结论，槲皮素保护酵母pep4突变细胞免受氧化应激和凋亡，从而增加在时间老化过程中的生存能力。
The yeast Saccharomyces cerevisiae PEP4 gene encodes vacuolar endopeptidase proteinase A (Pep4p), which is a homolog of the human CTSD gene that encodes cathepsin D. Mutation of CTSD gene in human resulted in a number of neurodegenerative diseases. In this study, we have shown that yeast pep4 mutant cells are highly sensitive to oxidative and apoptotic stress induced by hydrogen peroxide and acetic acid, respectively. pep4a dagger cells also showed accumulation of reactive oxygen species (ROS), apoptotic markers, and reduced chronological lifespan. In contrast, quercetin pretreatment protected the pep4 mutant from oxidative and apoptotic stress-induced sensitivity by scavenging ROS and reducing apoptotic markers. The percentage viability of quercetin-treated pep4a dagger cells was more pronounced and increased stress resistance against oxidant, apoptotic, and heat stress during chronological aging. From our experimental results, we concluded that quercetin protects yeast pep4 mutant cells from oxidative stress and apoptosis, thereby increasing viability during chronological aging.