Curcumin induced suicidal erythrocyte death
Curcumin induced suicidal erythrocyte death
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DOI：
10.1159/000099203
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Lang, Florian
中科院分区：
文献类型：
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作者：
Bentzen, Peter J.;Lang, Elisabeth;Lang, Florian
具有抗炎和抗肿瘤活性的天然营养成分姜黄素此前已被证明可以刺激多种有核细胞类型的细胞凋亡。本研究旨在探讨姜黄素是否可以类似地诱导红细胞自杀性死亡或红细胞凋亡，红细胞凋亡的特征是红细胞表面的磷脂酰丝氨酸暴露导致细胞收缩和细胞膜混乱。磷脂酰丝氨酸暴露细胞被吞噬，从而从循环血液中迅速清除。红细胞膜紊乱可能是由胞质Ca 2+活性增加或神经酰胺形成引起的。为了检测红细胞凋亡，根据膜联蛋白V结合估计红细胞磷脂酰丝氨酸暴露，并根据FACS分析中的前向散射估计红细胞体积。红细胞暴露于姜黄素（= 1 μ M）增加膜联蛋白V结合和减少前向散射，指向磷脂酰丝氨酸暴露在细胞表面和细胞收缩。根据Fluo 3荧光姜黄素增加胞质Ca 2+的活性，并根据免疫荧光姜黄素增加神经酰胺的形成。如前所述，高渗休克（添加550 mM蔗糖）、氯化物去除和葡萄糖消耗降低了前向散射并增加了膜联蛋白V结合。姜黄素的存在下，对膜联蛋白结合的影响增强。然而，接触姜黄素并没有显著增强高渗休克或Cl-去除的收缩效应，并逆转了葡萄糖戒断的收缩效应。本观察公开了姜黄素的促凋亡作用，其可影响循环红细胞的寿命。版权所有（c）2007 S. Karger AG，巴塞尔。
The natural nutrient component Curcumin with antiinflammatory and antitumor activity has previously been shown to stimulate apoptosis of several nucleated cell types. The present study has been performed to explore whether Curcumin could similarly induce suicidal death of erythrocytes or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. Phosphatidylserine exposing cells are phagocytosed and thus rapidly cleared from circulating blood. Erythrocyte membrane scrambling may be triggered by increase of cytosolic Ca2+ activity or formation of ceramide. To test for eryptosis, erythrocyte phosphatidylserine exposure has been estimated from annexin V binding, and erythrocyte volume from forward scatter in FACS analysis. Exposure of erythrocytes to Curcumin (= 1 mu M) increased annexin V binding and decreased forward scatter, pointing to phosphatidylserine exposure at the cell surface and cell shrinkage. According to Fluo3 fluorescence Curcumin increased cytosolic Ca2+ activity and according to immunofluorescence Curcumin increased ceramide formation. As shown previously, hypertonic shock (addition of 550mM sucrose), chloride removal and glucose depletion decreased the forward scatter and increased annexin V binding. The effects on annexin binding were enhanced in the presence of Curcumin. Exposure to Curcumin did, however, not significantly enhance the shrinking effect of hypertonic shock or Cl- removal and reversed the shrinking effect of glucose withdrawal. The present observations disclose a proeryptotic effect of Curcumin which may affect the life span of circulating erythrocytes. Copyright (c) 2007 S. Karger AG, Basel.