Quantitative trait loci associated with maximal exercise endurance in mice
Quantitative trait loci associated with maximal exercise endurance in mice
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DOI：
10.1152/japplphysiol.01328.2006
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发表时间：
2007-07-01
影响因子：
3.3
通讯作者：
Kleeberger, Steven R.
中科院分区：
文献类型：
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作者：
Lightfoot, J. Timothy;Turner, Michael J.;Kleeberger, Steven R.
遗传学在决定最大运动耐力中的作用尚不清楚。6至9周龄的F1小鼠（n = 99; 60只雌性，39只雄性），来自两个近交系的杂交，所述近交系先前被表型分型为具有高最大运动耐力（Balb/cJ）和低最大运动耐力（DBA/2 J），进行跑步机测试以估计运动耐力。对F-2组群（n = 12高运动耐力; n = 12低运动耐力）的选择性基因分型在整个组群中在X染色体（53.7 cM，DXMit 121）上鉴定出显著的数量性状位点（QTL），并且在雌性小鼠中在8号染色体（36.1 cM，D8 Mit 359）上鉴定出暗示性QTL。用整个F群和额外的信息标记进行精细作图证实并缩小了QTL。8号染色体QTL（EE 8（F））与两个提示性的人类QTL和一个先前与运动耐力连锁的显著的大鼠QTL同源。在F2队列中未发现性别（P = 0.33）或体重（P = 0.79）对运动耐力的影响。这些数据表明，遗传因素在不同的染色体区域可能会影响最大运动耐力的近交系小鼠。而多个基因位于所确定的QTL，可以在功能上影响运动耐力，这项研究作为进一步调查的基础，描绘的身份影响最大运动耐力的遗传因素。
The role of genetics in the determination of maximal exercise endurance is unclear. Six- to nine-week-old F, mice (n = 99; 60 female, 39 male), derived from an intercross of two inbred strains that had previously been phenotyped as having high maximal exercise endurance (Balb/cJ) and low maximal exercise endurance (DBA/2J), were treadmill tested to estimate exercise endurance. Selective genotyping of the F-2 cohort (n = 12 high exercise endurance; n = 12 low exercise endurance) identified a significant quantitative trait locus (QTL) on chromosome X (53.7 cM, DXMit121) in the entire cohort and a suggestive QTL on chromosome 8 (36.1 cM, D8Mit359) in the female mice. Fine mapping with the entire F, cohort and additional informative markers confirmed and narrowed the QTLs. The chromosome 8 QTL (EE8(F)) is homologous with two suggestive human QTLs and one significant rat QTL previously linked with exercise endurance. No effect of sex (P = 0.33) or body weight (P = 0.79) on exercise endurance was found in the F2 cohort. These data indicate that genetic factors in distinct chromosomal regions may affect maximal exercise endurance in the inbred mouse. Whereas multiple genes are located in the identified QTL that could functionally affect exercise endurance, this study serves as a foundation for further investigations delineating the identity of genetic factors influencing maximum exercise endurance.