Function of the Drosophila CPEB protein Orb2 in long-term courtship memory
Function of the Drosophila CPEB protein Orb2 in long-term courtship memory
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DOI：
10.1038/nn1996
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发表时间：
2007-12-01
影响因子：
25
通讯作者：
Dickson, Barry J.
中科院分区：
文献类型：
--
作者：
Keleman, Krystyna;Kruettner, Sebastian;Dickson, Barry J.
长期行为记忆和突触可塑性都需要蛋白质合成，其中一些可能发生在特定突触的局部。细胞质聚腺苷化元件结合（CPEB）蛋白被认为有助于局部蛋白质合成，从而导致突触效能的长期变化，但它们在长期行为记忆形成中的作用尚未确定。我们发现黑腹果蝇CPEB蛋白Orb2在雄性求偶行为的长期调节中是非常需要的。Orb2的n端谷氨酰胺丰富区域的缺失导致果蝇形成长期记忆的能力受损，而不是短期记忆。通过选择性地在蘑菇体无果（fru）阳性c神经元中表达Orb2，以及仅在训练期间和训练后不久在蘑菇体中提供Orb2功能来恢复记忆。因此，我们的数据表明，CPEB蛋白在长期记忆中很重要，并绘制了其在记忆形成中的功能的分子，空间和时间要求。
Both long-term behavioral memory and synaptic plasticity require protein synthesis, some of which may occur locally at specific synapses. Cytoplasmic polyadenylation element-binding (CPEB) proteins are thought to contribute to the local protein synthesis that underlies long-term changes in synaptic efficacy, but a role has not been established for them in the formation of long-term behavioral memory. We found that the Drosophila melanogaster CPEB protein Orb2 is acutely required for long-term conditioning of male courtship behavior. Deletion of the N-terminal glutamine-rich region of Orb2 resulted in flies that were impaired in their ability to form long-term, but not short-term, memory. Memory was restored by expressing Orb2 selectively in fruitless (fru)positive c neurons of the mushroom bodies and by providing Orb2 function in mushroom bodies only during and shortly after training. Our data thus demonstrate that a CPEB protein is important in long-term memory and map the molecular, spatial and temporal requirements for its function in memory formation.