IDENTIFICATION OF AN RFLP MARKER TIGHTLY LINKED TO THE HT1 GENE IN MAIZE
IDENTIFICATION OF AN RFLP MARKER TIGHTLY LINKED TO THE HT1 GENE IN MAIZE
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DOI：
10.1007/bf00588588
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发表时间：
1991-01-01
影响因子：
5.4
通讯作者：
FREYSSINET, G
中科院分区：
文献类型：
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作者：
BENTOLILA, S;GUITTON, C;FREYSSINET, G
我们已经确定了紧密连锁的限制性片段长度多态性标记的Ht 1基因的玉米，赋予抗真菌病原体大斑病菌种族1。 这是通过使用四对近等基因系（近等基因系; B73，A619，W153 R，和CM 105），每个不同的基因Ht 1的存在或不存在。 由于Ht 1映射到2号染色体，26个克隆已经映射到该染色体标记和探测对这些近等离子体DNA消化三种限制性内切酶：EcoRI，BamHI，和HindIII的Southern印迹。 有6个标记至少与一对近等基因系存在RFLP。 通过分析6个标记中的5个（其中1个在所研究的杂交中为单态）的分离和对H.在DF 20 × LH 146 Ht的95个F_2代个体上检测到turcicum 1号生理小种。 结果表明，一个紧密的连锁之间的DNA标记，UMC 150 B，和Ht 1基因。
We have identified tight linkage of an RFLP marker to the Ht1 gene of maize that confers resistance to the fungal pathogen Helminthosporium turcicum race 1. This was accomplished by the use of four pairs of near isogenic lines (NILs; B73, A619, W153R, and CM105), each differing by the presence or the absence of the gene Ht1. Since Ht1 maps to chromosome 2, 26 clones already mapped to this chromosome were labeled and probed against Southern blots of these NILs DNA digested with three restriction enzymes: EcoRI, BamHI, and HindIII. Six markers exhibited an RFLP for at least one pair of NILs. Presumptive linkage was further tested by analyzing the segregation of five of the six markers (one was monomorphic in the cross studied) and resistance to H. turcicum race 1 on 95 F2 individuals from the cross DF20 x LH146Ht. The results indicate a tight linkage between one of the DNA markers, UMC150B, and the Ht1 gene.